| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Outline of Final Research Achievements |
Phenolic compounds are known to be efficient antioxidants; however, their use is often limited by their weak solubility either in hydrophilic or hydrophobic formulations. Thus, glycosylation of hydrophilic compound can be a potent strategy to alter their solubility. In the present study, we synthesized new phenol compounds using rutinase as transglycosidative enzyme and investigated its neuroprotective properties. Rutinosides were prepared using the reverse reaction of hydrolysis by rutinase. More than 10 kinds of phenolic compounds were successfully glycosylated by the reverse reaction of rutinase. The solubility and Caco-2 permeability of ferulic acid rutinoside (FAR) significantly improved by glycosylation with rutinose. FAR induced the upregulation of neuroprotection-related genes such as BDNF and NGF in mouse primary astrocytes. These results suggest that transglycosylation using rutinose is an effective method for preparing novel neuroprotective phenolic compounds.
|
