Development of Antioxidative Absorption Capacity Assay Method for Foods

• Project/Area Number: 15K07431
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Ehime University
• Principal Investigator: Nagaoka Shin-ichi 愛媛大学, 理工学研究科(理学系), 教授 (30164403)
• Co-Investigator(Renkei-kenkyūsha): OHARA Keishi 愛媛大学, 大学院理工学研究科, 教授 (10284390)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 食品機能

Outline of Final Research Achievements

An aroxyl radical absorption capacity assay-method (ARAC assay method), a singlet oxygen absorption capacity assay-method (SOAC assay method), and an alfa-tocopherol-recycling capacity assay-method (ATREC assay method) were developted for various antioxidants and foods, such as flavones, anthocyanidines, hydroxyanthraquinones, unsaturated fatty acids, carotenoids, catecholamines, pyrroloquinoline quinol, catechins, vegitable oils, rice bran extracts, and so on. The aroxyl radical is a model of free radical, which is, as well as singlet oxygen, one of the reactive oxygen species. The data obtained here are summarized as a database and avialable as AbsorptionCapacity.accdb on our website at http://chem.sci.ehime-u.ac.jp/~struct1/int_naga.pdf.

Research Products

• [Journal Article] Kinetic Study of the Aroxyl-Radical-Scavenging Activity of Five Fatty Acid Esters and Six Carotenoids in Toluene Solution: Structure: Activity Relationship for the Hydrogen Abstraction Reaction (2017)
  • Author(s): Mukai Kazuo、Yoshimoto Maya、Ishikura Masaharu、Nagaoka Shin-ichi
  • Journal Title: J. Phys. Chem. B Volume: 121 Issue: 32 Pages: 7593-7601
  • DOI: 10.1021/acs.jpcb.7b04570
  • Peer Reviewed
• [Journal Article] Correlation among Singlet-Oxygen Quenching, Free-Radical Scavenging, and Excited-State Intramolecular-Proton-Transfer Activities in Hydroxyflavones, Anthocyanidins, and 1-Hydroxyanthraquinones (2017)
  • Author(s): Nagaoka Shin-ichi, Bandoh Yuki, Nagashima Umpei, Ohara Keishi
  • Journal Title: JOURNAL OF PHYSICAL CHEMISTRY A Volume: 121 Issue: 42 Pages: 8069-8079
  • DOI: 10.1021/acs.jpca.7b07869
  • Peer Reviewed
• [Journal Article] Practical Training on Adding Polarization Function to Basis Set for Molecular Orbital Calculation of Ethylene (2017)
  • Author(s): Nagaoka Shin-ichi、Teramae Hiroyuki、Nagashima Umpei
  • Journal Title: Journal of Computer Chemistry, Japan -International Edition Volume: 3 Issue: 0 Pages: n/a
  • DOI: 10.2477/jccjie.2016-0068
  • NAID: 130007041797
  • ISSN: 2189-048X
  • Peer Reviewed / Open Access
• [Journal Article] Development of Singlet Oxygen Absorption Capacity (SOAC) Assay Method. Measurements of the SOAC Values for Carotenoids and α-Tocopherol in Aqueous Triton X-100 Micellar Solution (2017)
  • Author(s): K. Mukai, A. Ouchi, N. Azuma, S. Takahashi, K. Aizawa, and S. Nagaoka
  • Journal Title: J. Agric. Food Chem. Volume: 65 Issue: 4 Pages: 784-792
  • DOI: 10.1021/acs.jafc.6b04329
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Tunneling Effect in Vitamin E Recycling by Green Tea (2016)
  • Author(s): S. Nagaoka, A. Nitta, A. Suemitsu, and K. Mukai
  • Journal Title: RSC Adv. Volume: 6 Issue: 53 Pages: 47325-47336
  • DOI: 10.1039/c6ra05986d
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Kinetic Study of Aroxyl-Radical-Scavenging and α-Tocopherol-Regeneration Rates of Five Catecholamines in Solution: Synergistic effect of α-Tocopherol and Catecholamines (2016)
  • Author(s): K. Mukai, K. Nagai, Y. Egawa, A. Ouchi, S. Nagaoka
  • Journal Title: J. Phys. Chem. B Volume: 120 Issue: 29 Pages: 7088-7097
  • DOI: 10.1021/acs.jpcb.6b04285
  • Peer Reviewed
• [Journal Article] ビタミンEの一重項酸素消去速度に及ぼす金属塩の効果 (2016)
  • Author(s): 長岡伸一、高橋京香、中村明日香、小原敬士、向井和男
  • Journal Title: ビタミンE研究の進歩 Volume: XVII Pages: 53-56
• [Journal Article] Pyrroloquinoline Quinone (PQQ) Is Reduced to Pyrroloquinoline Quinol (PQQH2) by Vitamin C, and PQQH2 Produced is Recycled to PQQ by Air Oxidation in Buffer Solution at pH 7.4 (2016)
  • Author(s): K. Mukai, A. Ouchi, S. Nagaoka, M. Nakano, and K. Ikemoto
  • Journal Title: Biosci. Biotech. Biochem. Volume: 80 Issue: 1 Pages: 178-187
  • DOI: 10.1080/09168451.2015.1072462
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] TD DFT Study of Intramolecular Proton Transfer by Absorption And Emission Spectra of o-Hydroxybenzaldehyde (2016)
  • Author(s): 新井健文、長岡伸一、長嶋雲兵、寺前裕之
  • Journal Title: Journal of Computer Chemistry, Japan Volume: 14 Issue: 6 Pages: 209-210
  • DOI: 10.2477/jccj.2015-0061
  • NAID: 130005125141
  • ISSN: 1347-1767, 1347-3824
  • Peer Reviewed / Open Access
• [Journal Article] アロエ含有物によるUVケアの研究－紫外線防御と一重項酸素消去作用 (2015)
  • Author(s): 長岡伸一、小原敬士
  • Journal Title: フレグランスジャーナル Volume: 43(6) Pages: 67-72
  • NAID: 40019435246
  • Acknowledgement Compliant
• [Journal Article] A Study of UV Care by Molecules Contained in Aloe - UV Protection and Singlet-Oxygen Quenching (2015)
  • Author(s): 長岡伸一、小原敬士
  • Journal Title: Fragrance J. Korea Volume: 2015-7 Pages: 88-93
  • NAID: 40020512126
  • Acknowledgement Compliant
• [Journal Article] Notable Effects of the Metal Salts on the Quenching Reaction of Singlet Oxygen by α-Tocopherol in Ethanol Solution (2015)
  • Author(s): K. Mukai, A. Nakamura, S. Nagaoka, A. Ouchi, and N. Azuma
  • Journal Title: Bulletin of the Chemical Society of Japan Volume: 88 Issue: 10 Pages: 1503-1510
  • DOI: 10.1246/bcsj.20150182
  • NAID: 130005102816
  • ISSN: 0009-2673, 1348-0634
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Kinetic Study of the Quenching Reaction of Singlet Oxygen by Seven Rice Bran Extracts in Ethanol Solution. Development of a Singlet Oxygen Absorption Capacity (SOAC) Assay Method (2015)
  • Author(s): K. Mukai, E. Ishikawa, T. Abe, A. Ouchi, S. Nagaoka, K. Murata, T. Miyazawa, and K. Nakagawa
  • Journal Title: Biosci. Biotech. Biochem. Volume: 79 Issue: 12 Pages: 2063-2072
  • DOI: 10.1080/09168451.2015.1069701
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Kinetic Study of the Scavenging Reaction of the Aroxyl Radical by Eight Kinds of Vegetable Oils in Solution. Development of an Aroxyl Radical Absorption Capacity (ARAC) Assay Method (2017)
  • Author(s): K. Mukai, Y. Bandoh, S. Nagaoka, H. Itoh, E. Kobayashi, K. Nakagawa
  • Organizer: The Asian Conference Oleo Science 2017 & the 56th Annual Meeting of the Japan Oil Chemists' Society
  • Int'l Joint Research
• [Presentation] 化学反応を制御する一つの方法 (2017)
  • Author(s): 長岡伸一
  • Organizer: 日本真空学会 2017年9月研究例会
  • Invited
• [Presentation] H3+、H3、H3-の結合様式と構造 (2017)
  • Author(s): 長嶋雲兵、田島澄恵、中山尚史、寺前裕之、長岡伸一
  • Organizer: 日本コンピュータ化学会2017秋季年会
• [Presentation] 野菜抽出物によるフリーラジカル消去活性評価法の開発 (2016)
  • Author(s): 坂東宥奎、川田耕司、向井和男、櫻井康博、服部玄、倉中綾、長岡伸一
  • Organizer: 第55回電子スピンサイエンス学会
  • Place of Presentation: 大阪市立大学（杉本キャンパス） （大阪府・大阪市）
  • Year and Date: 2016-11-10
• [Presentation] 一重項酸素消去活性評価法 (SOAC法) の開発： Triton X-100 ミセル溶液中におけるカロテノイド類とビタミンＥのSOAC 値の評価 (2016)
  • Author(s): 向井和男、大内綾、東長雄、高橋慎吾、相澤宏一、長岡伸一
  • Organizer: 第55回電子スピンサイエンス学会
  • Place of Presentation: 大阪市立大学（杉本キャンパス） （大阪府・大阪市）
  • Year and Date: 2016-11-10
• [Presentation] シコニン・ヒドロキシナフトキノン類の一重項酸素消去活性と励起状態分子内水素移動 (2016)
  • Author(s): 小原敬士、古信裕、尾下愛美、長岡伸一
  • Organizer: 2016年日本化学会中国四国支部大会香川大会
  • Place of Presentation: 香川大学幸町キャンパス （香川県・高松市）
  • Year and Date: 2016-11-05
• [Presentation] CORRELATION BETWEEN ANTIOXIDANT AND EXCITED-STATE INTRAMOLECULAR-PROTON-TRANSFER ACTIVITIES IN ANTHOCYANIDINS AND HYDROXYANTHRAQUINONES (2016)
  • Author(s): Yuki Bandoh, Keishi Ohara, and Shin-ichi Nagaoka
  • Organizer: 26th IUPAC International Symposium on Photochemistry
  • Place of Presentation: Osaka City Central Public Hall (大阪府・大阪市)
  • Year and Date: 2016-04-03
  • Int'l Joint Research
• [Presentation] ビタミンEの一重項酸素消去速度に及ぼす金属塩の効果 (2016)
  • Author(s): 長岡伸一、髙橋京香、中村明日香、小原敬士、向井和男
  • Organizer: 第27回ビタミンE研究会
  • Place of Presentation: アルファあなぶきホール(香川県・高松市)
  • Year and Date: 2016-01-08
• [Presentation] 近赤外発光スポット検出によるゲル中の一重項酸素寿命計測 (2015)
  • Author(s): 小原敬士、北平有望、長岡伸一
  • Organizer: 2015年日本化学会中国四国支部大会
  • Place of Presentation: 岡山大学(岡山県・岡山市)
  • Year and Date: 2015-11-14
• [Presentation] TD DFT法によるOHBAの吸光・発光スペクトルの理論的研究 (2015)
  • Author(s): 新井健文、長岡伸一、長嶋雲兵、寺前裕之
  • Organizer: 日本コンピューター化学会2015秋季年会
  • Place of Presentation: 函館市まちづくりセンター (北海道・函館市)
  • Year and Date: 2015-10-30
• [Presentation] Nodal-Plane Model in Photochemistry (2015)
  • Author(s): 長岡 伸一、小原 敬士
  • Organizer: 27th International Conference on Photochemistry
  • Place of Presentation: Jeju Island, Korea
  • Year and Date: 2015-06-28
  • Int'l Joint Research / Invited
• [Remarks] ARAC値、SOAC値のデータベース
  • URL: http://chem.sci.ehime-u.ac.jp/~struct1/int_naga.pdf
• [Remarks] AbsorptionCapacity.accdb
  • URL: http://chem.sci.ehime-u.ac.jp/~struct1/int_naga.pdf