| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Lysozyme is widely distributed in various biological fluids and tissues. The effect of lysozyme on macrophages involved in inflammatory responses was examined. Lysozyme significantly suppressed the production of TNF-α and IL-6 by LPS-treated mouse macrophage cell line RAW264.7 cells and mouse peritoneal macrophages in a dose-dependent manner due to suppression of the gene expression levels of these cytokines. Zymosan A-mediated phagocytosis activity was not affected by lysozyme, suggesting that lysozyme shows the anti-inflammatory effect without inhibiting the phagocytotic response of macrophages. In addition, lysozyme inhibited phosphorylation of c-Jun N-terminal kinase (JNK). It is suggested that lysozyme intracellularly suppresses LPS-induced inflammatory response by inhibiting JNK phosphorylation in macrophages. Oral administration of lysozyme significantly suppressed the serum IL-6 and TNF-α levels in LPS-induced inflammation model mice.
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