| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The production of prebiotics for intestinal bifidobacteria from two food wastes was studied.
(1) By using bifidobacterial glycosidases or cells, bifidogenic disaccharide galacto-N-biose was released from wasted mucin, which contains abundant O-glycans. A novel 6-sulfo-N-acetylglucosaminidase, an essential enzyme for degradation of terminally sulfated glycans, was identified. A bifidobacterial strain defective in incorporation of galacto-N-biose was also screened, which is suitable for production of the disaccharide.
(2) Mume-polyphenols prepared from umesu, a waste of umeboshi production, was found to enhance bifidobacterial growth. The major phenolic compound, prunose, was assimilated by B. catenulatum and B. pseudocatenulatum, which express prunose-specific ferulate esterase. Arabinan-like polysaccharides was degraded by two novel GH43 exo-α-arabinofuranosidases from B. longum. Effects of orally administrated mume-polyphenols on intestinal microbiota was evaluated using mice.
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