Evaluation of cytotoxicity by Surface Plasmon Resonance (SPR) sensor using cell-immobilized sensor chip

Research Project

Project/Area Number	15K07457
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Food science
Research Institution	Fukuoka Women's University
Principal Investigator	KOBAYASHI Hiroshi 福岡女子大学, 国際文理学部, 准教授 (00610255)
Co-Investigator(Kenkyū-buntansha)	石川洋哉福岡女子大学, 国際文理学部, 准教授 (00325490)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	細胞固定化センサ / 毒性評価 / SPR / 細胞毒性 / 細胞培養 / ベロ毒素 / SPRセンサ / 細胞毒性評価
Outline of Final Research Achievements	In this research, we tried to develop a method for evaluating cytotoxicity by Surface Plasmon Resonance (SPR) sensor using cell-immobilized sensor chip. As a result, 1) a method of immobilizing cells on the sensor chip gold film was constructed, 2) the response of the cell to the verotoxin (macromolecule) can be measured as a SPR signal change, 3) the cell hydrogen peroxide (Low molecular weight) can be measured as SPR signal change.

Report

(4 results)