Evaluation of cytotoxicity by Surface Plasmon Resonance (SPR) sensor using cell-immobilized sensor chip
| Project/Area Number |
15K07457
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Fukuoka Women's University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
石川 洋哉 福岡女子大学, 国際文理学部, 准教授 (00325490)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 細胞固定化センサ / 毒性評価 / SPR / 細胞毒性 / 細胞培養 / ベロ毒素 / SPRセンサ / 細胞毒性評価 |
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| Outline of Final Research Achievements |
In this research, we tried to develop a method for evaluating cytotoxicity by Surface Plasmon Resonance (SPR) sensor using cell-immobilized sensor chip. As a result, 1) a method of immobilizing cells on the sensor chip gold film was constructed, 2) the response of the cell to the verotoxin (macromolecule) can be measured as a SPR signal change, 3) the cell hydrogen peroxide (Low molecular weight) can be measured as SPR signal change.
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Report
(4 results)
Research Products
(1 results)
