| Project/Area Number |
15K07459
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Toyo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
木村 真 名古屋大学, 生命農学研究科, 准教授 (20261167)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | カビ毒 / トリコテセン / ニバレノール / ELISA / 食の安全 / 生合成酵素 / 異種発現 |
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| Outline of Final Research Achievements |
Filamentous fungi such as Fusarium graminearum produce trichothecene mycotoxins including nivalenol (NIV). However, since effective antibodies necessary for ELISA to detect NIV-type trichothecenes have not been obtained yet, these mycotoxins are not regulated properly. Thus, we assumed that NIV-type trichothecenes can be converted to 3,4,15-triacetylnivalenol by acetylase, and this product can be measured by ELISA. In this study, we attempted to acquire enzymes that acetylate at positions C-3, C-4, and C-15 of NIV-type trichothecenes, from F. graminearum we purchased and modified by genetic engineering, and soil microorganisms we screened for. We succeeded in finding the proper conditions for collective conversion of NIV-type trichothecenes to 3,4,15-triacetylnivalenol, which is detectable by ELISA. This research may contribute to the construction of a collective detection system of NIV-type trichothecenes.
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