Technology development to acetylate nivalenol-type trichothecenes to tri-acetylnivalenol for a collective detection method
| Project/Area Number |
15K07459
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Toyo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
木村 真 名古屋大学, 生命農学研究科, 准教授 (20261167)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | カビ毒 / トリコテセン / ニバレノール / ELISA / 食の安全 / 生合成酵素 / 異種発現 |
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| Outline of Final Research Achievements |
Filamentous fungi such as Fusarium graminearum produce trichothecene mycotoxins including nivalenol (NIV). However, since effective antibodies necessary for ELISA to detect NIV-type trichothecenes have not been obtained yet, these mycotoxins are not regulated properly. Thus, we assumed that NIV-type trichothecenes can be converted to 3,4,15-triacetylnivalenol by acetylase, and this product can be measured by ELISA. In this study, we attempted to acquire enzymes that acetylate at positions C-3, C-4, and C-15 of NIV-type trichothecenes, from F. graminearum we purchased and modified by genetic engineering, and soil microorganisms we screened for. We succeeded in finding the proper conditions for collective conversion of NIV-type trichothecenes to 3,4,15-triacetylnivalenol, which is detectable by ELISA. This research may contribute to the construction of a collective detection system of NIV-type trichothecenes.
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Report
(3 results)
Research Products
(27 results)
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[Journal Article] Exploring an artificial metabolic route in Fusarium sporotrichioides: Production and characterization of 7-Hydroxy T-2 toxin.2018
Author(s)
KAMATA, K., SATO, H., MAEDA, K., FURIHATA, K., AIKAWA, S., ADACHI, K., TANAKA, A., TOKAI, T., NAKAJIMA, Y., YOSHIDA, Y., SAKUDA, S., KIMURA, M. and TAKAHASHI-ANDO, N.
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Journal Title
J. Nat. Prod.
Volume: 印刷中
Issue: 4
Pages: 1041-1044
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Accumulation of an unusual trichothecene shunt metabolite in liquid culture of <i>Fusarium graminearum</i> with methionine as the sole nitrogen source2017
Author(s)
NAKAJIMA, Y., TANAKA, Y., MATSUI, K., MAEDA, K., KITOU, Y., KANAMARU, K., OHSATO, S., KOBAYASHI, T., TAKAHASHI-ANDO, N., KIMURA, M.
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Journal Title
JSM Mycotoxins
Volume: 67
Issue: 1
Pages: 7-9
DOI
NAID
ISSN
0285-1466, 1881-0128
Related Report
Peer Reviewed / Open Access
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