Development of systematic methods for safety assurance of genotoxicity mycotoxins

• Project/Area Number: 15K07462
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Azabu University
• Principal Investigator: KONISHI YOSHIKO 麻布大学, 生命・環境科学部, 教授 (10195761)
• Co-Investigator(Renkei-kenkyūsha): UEDA HIROSHI 東京工業大学, 資源化学研究所, 教授 (60232758)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 食品安全性 / 遺伝毒性物質 / アフラトキシン / デトックス / 遺伝毒性 / バイオマーカー / オープンサンドイッチELISA / アフラトキシンM1 / デトキシケーション / ELISA / AFM1の吸着能 / Lactococcus lactis / モノクローナル抗体 / アフラトキシンＭ１

Outline of Final Research Achievements

Aflatoxins (AFs) are genotoxic mycotoxin and　FAO/WHO recommends that AFs not take as much as possible. In this study, the systematic methods how to minimize the intake of AFs were developed. we developed noncompetitive homogeneous ELISA with the information of DNA sequence of monoclonal antibody to measure AFs in food. We found that Lactococcus lactis subsp. lactis NCDO 604T isolated from cucumber had high affinity to AFs as a detoxication of AFs.We found the biomarker to monitor of exposure to AFB1. The mouse in vivo experiment revealed that AFM1 in urine was good biomarker within 24hrs post intake of AFB1. Exposure to AFs could be suppressed by using these methods systematically.

Research Products

• [Journal Article] Aflatoxin B1 Contamination of Dairy Feeds after Storage in Farm Practice in Tropical Environmen (2017)
  • Author(s): WANTANWA MONGKON, YOSHIKO SUGITA-KONISHI, WASANA CHAISRI, WITAYA SURIYASATHAPORN
  • Journal Title: Biocontrol Science Volume: 22 Issue: 1 Pages: 41-45
  • DOI: 10.4265/bio.22.41
  • NAID: 130005493107
  • ISSN: 1342-4815, 1884-0205
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Feed and feed storage factors in relation to aflatoxin M1 (2017)
  • Author(s): Wasana Chaisri, Wantanwa Mongkon, Yoshiko Sugita-Konishi, ,Dirk Van Dam, Ingrid Huntle, Witaya Suriyasathaporn
  • Journal Title: JSM Mycotoxins Volume: 67(2) Pages: 85-88
  • Peer Reviewed / Open Access
• [Journal Article] Mycotoxin contamination in foodstuffs and feeds-health concerns in Thailand (2017)
  • Author(s): Phitsanu Tulayakul、Yoshiko Sugita-Konishi
  • Journal Title: Japanese Journal of Veterinary Research Volume: 印刷中
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Enrichment factors of aflatoxin M₁ in dairy products for making naturally and artificially aflatoxin M₁ contaminated milk (2016)
  • Author(s): Hisako Sakuma1, Yoichi Kamata, Yoshiko Sugita-Konishi,
  • Journal Title: Japanese Journal of Food Chemistry and Safety Volume: 23 Issue: 3 Pages: 118-125
  • DOI: 10.18891/jjfcs.23.3_118
  • NAID: 130005252679
  • ISSN: 1341-2094, 2189-6445
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 野菜由来乳酸菌のアフラトキシン類への結合能と胃内環境での挙動 (2018)
  • Author(s): 窪田祐恵、尾畑瑠衣、内藤千秋、大仲賢二、石崎直人、小林直樹、小西良子
  • Organizer: 日本マイコトキシン学会
• [Presentation] アフラトキシン結合能を有する野菜由来乳酸菌の探索と消化液での安定性に関する研究 (2017)
  • Author(s): 尾畑瑠衣、窪田祐恵、内藤千秋、大仲賢二、石崎直人、小林直樹、小西良子
  • Organizer: 第113回 日本食品衛生学会学術総会
• [Presentation] Feed and feed management factors in relationship to aflatoxin M1 contamination in bulk milk of small holder dairy farms. (2016)
  • Author(s): Wasana Chaisri、
  • Organizer: ISMYCO2016
  • Place of Presentation: 東京大学弥生ホール
  • Year and Date: 2016-11-30
  • Int'l Joint Research
• [Presentation] タイ国の牛乳を用いたin-houseおよび市販アフラトキシンM1 (2015)
  • Author(s): 小西良子
  • Organizer: 日本マイコトキシン学会
  • Place of Presentation: 庄内産業振興センター マリカ市民ホー
  • Year and Date: 2015-09-11