| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Characterization of channel and transporter genes involved in membrane transport of potassium (K) ion in sugi (Cryptomeria japonica) was conducted. Complementation assays using an Escherichia coli mutant deficient in K-ion uptake function demonstrated that all channels and transporters examined in this study have a function in K-ion uptake. Further assays indicated that K-ion uptake mediated by these channels and transporters is influenced by other cations, such as cesium and calcium ions. Expression analysis showed that all genes analyzed in this study were expressed in multiple parts of sugi trees. In male strobili, the expression of one transporter gene increased as the developmental stage proceeded. Conversely, the other genes were expressed higher in developing male strobili than in mature male strobili.
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