Study on plant cell wall lignin by 13CO2 administration

• Project/Area Number: 15K07510
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Wood science
• Research Institution: Nagoya University
• Principal Investigator: Aoki Dan 名古屋大学, 生命農学研究科, 講師 (80595702)
• Co-Investigator(Renkei-kenkyūsha): FUKUSHIMA Kazuhiko 名古屋大学, 大学院生命農学研究科, 教授 (80222256) ; MATSUSHITA Yasuyuki 名古屋大学, 大学院生命農学研究科, 准教授 (60335015)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 13C二酸化炭素 / NMR / リグニン / イチョウ / 質量分析 / 固体NMR

Outline of Final Research Achievements

In this study, the cultivation of plant samples under 13CO2 atmosphere was examined for supersensitive structural analyses. At first, the environmentally controlled growth chamber was designed and built up. Plant samples were cultivated under N2+O2+CO2 (in chamber), N2+O2+13CO2 (in chamber), or air for 2 months. The resultant plant samples had high 13C ratio and supersensitive analyses was achieved successfully.

Research Products

• [Journal Article] Distribution of coniferin in freeze-fixed stem of Ginkgo biloba L. by cryo-TOF-SIMS/SEM (2016)
  • Author(s): D. Aoki, Y. Hanaya, T. Akita, Y. Matsushita, M. Yoshida, K. Kuroda, S. Yagami, R. Takama, K. Fukushima
  • Journal Title: Sci. Rep. Volume: 6 Issue: 1 Pages: 1-9
  • DOI: 10.1038/srep31525
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Chemical Mapping of Cell Wall Components (2016)
  • Author(s): 青木弾、松下泰幸、福島和彦
  • Journal Title: JAPAN TAPPI JOURNAL Volume: 70 Issue: 3 Pages: 308-315
  • DOI: 10.2524/jtappij.1506
  • NAID: 130005154367
  • ISSN: 0022-815X, 1881-1000
  • Peer Reviewed
• [Journal Article] Lignification of ray parenchyma cells (RPCs) in the xylem of Phellodendron amurense Rupr.: quantitative and structural investigation by TOF-SIMS and thioacidolysis of laser microdissection cuts of RPCs (2016)
  • Author(s): P. Zheng, D. Aoki, Y. Matsushita, S. Yagami, Y. Sano, M. Yoshida, K. Fukushima
  • Journal Title: Holzforschung Volume: 印刷中 Issue: 7 Pages: 641-652
  • DOI: 10.1515/hf-2015-0120
  • Peer Reviewed
• [Journal Article] イメージング質量分析による植物細胞壁木化過程の研究 (2015)
  • Author(s): 青木弾, 福島和彦
  • Journal Title: 植物の生長調節 Volume: 50 Pages: 57-63
  • Peer Reviewed
• [Presentation] 選択的13C標識法と固体NMRを用いたHG-DHPの構造解析 (2017)
  • Author(s): 橋浦雅、野村健太、松下泰幸、寺島典二、青木弾、福島和彦
  • Organizer: 第62回リグニン討論会
• [Presentation] 木材における顕微レベルでの化学成分分析 (2016)
  • Author(s): 青木弾
  • Organizer: 第46回木材の化学加工研究会シンポジウム
  • Place of Presentation: 山形
  • Year and Date: 2016-09-29
  • Invited
• [Presentation] Analyses of freeze-fixed plant samples by cryo-TOF-SIMS (2016)
  • Author(s): D. Aoki, Y. Matsushita, K. Kuroda, and K. Fukushima
  • Organizer: SISS-18
  • Place of Presentation: 武蔵野
  • Year and Date: 2016-07-21
  • Int'l Joint Research / Invited
• [Presentation] Chemical mapping of coniferin and mono/di-saccharides in freeze-fixed Ginkgo biloba by cryo time-of-flight secondary ion mass spectrometry (2016)
  • Author(s): D. Aoki, Y. Hanaya, T. Akita, Y. Matsushita, M. Yoshida, K. Kuroda, S. Yagami, R. Takama, K. Fukushima
  • Organizer: International Conference on Polyphenols 2016
  • Place of Presentation: Vienna
  • Year and Date: 2016-07-11
  • Int'l Joint Research
• [Presentation] 13C標識モノリグノール類を用いたリグニンの構造解析 (2016)
  • Author(s): 野村健太, 寺島典次, 松下泰幸, 青木弾, 西村裕志, 渡辺隆司, 片平正人, 福島和彦
  • Organizer: 第66回日本木材学会大会
  • Place of Presentation: 名古屋大学（愛知県名古屋市）
  • Year and Date: 2016-03-27
• [Presentation] Chemical Mapping of Plant Biomolecules by cryo Time-of-Flight Secondary Ion Mass Spectrometry (2015)
  • Author(s): Dan Aoki, Yuto Hanaya, Yasuyuki Matsushita, Masato Yoshida, Katsushi Kuroda, Ruka Takama, and Kazuhiko Fukushima
  • Organizer: 10th International Symposium on Atomic Level Characterizations for New Materials and Devices ’15
  • Place of Presentation: くにびきメッセ（島根県松江市）
  • Year and Date: 2015-10-25
  • Int'l Joint Research
• [Presentation] Chemical mapping of lignin precursors in the xylem of freeze-fixed Ginkgo biloba by the cryo-TOF-SIMS/SEM system (2015)
  • Author(s): K. Fukushima, Y. Hanaya, D. Aoki, Y. Matsushita, K. Kuroda
  • Organizer: 18th International Symposium on Wood, Fibre and Pulping Chemistry
  • Place of Presentation: Vienna (Austria)
  • Year and Date: 2015-09-09
  • Int'l Joint Research
• [Presentation] New method to divide xylem into compound middle lamella rich fraction and secondary wall rich fraction (2015)
  • Author(s): K. Nomura, N. Terashima, Y. Matsushita, D. Aoki, H. Nishimura, T. Watanabe, M. Katahira, and K. Fukushima
  • Organizer: The 6th International Symposium of Advanced Energy Science －Towards the Realization of Zero-Emission Energy－
  • Place of Presentation: 京都大学（京都府宇治市）
  • Year and Date: 2015-09-01
  • Int'l Joint Research
• [Presentation] Chemical mapping of organic/inorganic chemicals in frozen-hydrated biological samples (2015)
  • Author(s): D. Aoki, Y. Hanaya, Y. Matsushita, M. Yoshida, K. Kuroda, R. Takama, and K. Fukushima
  • Organizer: SISS-17
  • Place of Presentation: 成蹊大学（東京都武蔵野市）
  • Year and Date: 2015-06-25
  • Int'l Joint Research
• [Book] 植物細胞壁実験法 (2016)
  • Author(s): 石井忠、石水毅、梅澤俊明、加藤陽治、岸本崇生、小西照子、松永俊朗、福島和彦、松下泰幸、青木弾 他
  • Total Pages: 403
  • Publisher: 弘前大学出版会
• [Book] Secondary Xylem Biology (2016)
  • Author(s): Yoon Soo Kim, Ryo Funada, Adya P. Singh, D. Aoki, Y. Matsushita, K. Fukushima et al.
  • Total Pages: 397
  • Publisher: Academic Press