| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Basidiospore germination rate of Rhizopogone roseolus was improved by using 1/5-diluted MMN agar media and fruiting bodies with beige-colored section. Salt-tolerant homokaryotic strains were isolated from the basidiospore suspension treated with ethylmethanesulfonate. Salt-tolerant heterokaryotic strains were obtained by crossing between the salt-tolerant homokaryotic strains. We have selected the strains showing two useful characteristics, i.e. sat tolerance and vigorous mycelial growth. Pinus thunbergii seedlings inoculated with the salt-tolerant strain of R. roseolus were tolerant against salt stress, unlike the P. thunbergii seedlings inoculated with the salt-sensitive one. The sodium content in P. thunbergii seedlings inoculated with R. roseolus was greater than that in P. thunbergii seedlings inoculated without R. roseolus, indicating that the ectomycorrhizas formed by R. roseolus did not inhibit sodium uptake in the host P. thunbergii seedlings.
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