Development of Rhizogon roseolus strains showing salt tolerance and vigorous mycelial growth characteristics in order to reconstruct coastal and forest regions
| Project/Area Number |
15K07512
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Wood science
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2015-10-21 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | ショウロ / 外生菌根菌 / 担子胞子発芽 / 突然変異 / 塩ストレス耐性 / ナトリウム取込み / クロマツ / 外生菌根 / 耐塩性 / ホモカリオン |
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| Outline of Final Research Achievements |
Basidiospore germination rate of Rhizopogone roseolus was improved by using 1/5-diluted MMN agar media and fruiting bodies with beige-colored section. Salt-tolerant homokaryotic strains were isolated from the basidiospore suspension treated with ethylmethanesulfonate. Salt-tolerant heterokaryotic strains were obtained by crossing between the salt-tolerant homokaryotic strains. We have selected the strains showing two useful characteristics, i.e. sat tolerance and vigorous mycelial growth. Pinus thunbergii seedlings inoculated with the salt-tolerant strain of R. roseolus were tolerant against salt stress, unlike the P. thunbergii seedlings inoculated with the salt-sensitive one. The sodium content in P. thunbergii seedlings inoculated with R. roseolus was greater than that in P. thunbergii seedlings inoculated without R. roseolus, indicating that the ectomycorrhizas formed by R. roseolus did not inhibit sodium uptake in the host P. thunbergii seedlings.
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] Transcriptional analysis of alcohol and aldehyde dehydrogenase gene families in Pholiota microspora, and estimation of their physiological roles2016
Author(s)
Sutthikhampa,S. Kawai, Y., Hayashi, M., Boonlue, S., Shimomura, N., Yamaguchi, T. and Aimi, T.
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Journal Title
Mushroom Science and Biotechnology
Volume: 24
Pages: 16-23
NAID
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Identification, characterization and expression of the second glucoamylase gene from Pholiota microspora2016
Author(s)
Zhu, G., Wan, J., Hayashi, M., Shimomura, N., Yamaguchi, T. and Aimi, T.
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Journal Title
Mushroom Science and Biotechnology
Volume: 24
Pages: 77-84
NAID
Related Report
Peer Reviewed
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[Presentation] Identification of causal genes of albino (white fruiting body) Grifola frondosa2017
Author(s)
Kawaguchi, N., Zhu, G., Hayashi, M., Shimomura, N., Yamaguchi, T. and Aimi, T.
Organizer
The Ubon Ratchathani University Research Conference
Related Report
Int'l Joint Research / Invited
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[Presentation] Clamp forming ability determines the morphology of the fruiting body of Mycoleptodonoides aitchisonii2016
Author(s)
Wada, T., Takase, Y., Hashimoto, S., Sugimura, S. and Shimomura, N.
Organizer
International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia, 2016
Place of Presentation
Daejeon (Korea)
Year and Date
2016-11-08
Related Report
Int'l Joint Research
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[Presentation] Identification of bacteria that promote basidiospore germination in Rhizopogon roseolus under high salt conditions2016
Author(s)
Onda, Y., Nakano, S., Hiraoka, R., Gao, Q., Aimi, T. and Shimomura, N.
Organizer
International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia, 2016
Place of Presentation
Daejeon (Korea)
Year and Date
2016-11-08
Related Report
Int'l Joint Research
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