Why can't mushrooms ferment ethanol ?

• Project/Area Number: 15K07514
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Wood science
• Research Institution: Tottori University
• Principal Investigator: AIMI TADANORI 鳥取大学, 農学部, 教授 (90264928)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: きのこ / エタノール / アルコール脱水素酵素 / ピルビン酸脱炭酸酵素 / アルデヒド脱水素酵素 / グリセルアルデヒドリン酸脱水素酵素 / ナメコ / 発現解析 / アルコール / パン酵母 / グリセルアルデヒド-3-リン酸デヒドロゲナーゼ / カルボキシン耐性 / ハイグロマイシン耐性 / デンプン / アミラーゼ / 子実体 / 菌糸体 / プロモーター / 遺伝子発現 / ピルビン酸脱水素酵素 / 芳香族化合物 / マンニトール-1- リン酸デヒドロゲナーゼ

Outline of Final Research Achievements

In order to answer the question "Why mushrooms cannot ferment ethanol?", i)pyruvate decarboxylase, ii) alcohol dehydrogenase, and iii) aldehyde dehydrogenase genes which are key enzymes of alcohol production, were identified and their expression were analyzed.As the result, the expression of pyruvate decarboxylase gene is very weak, and it was strongly suggested that the role of alcohol dehydrogenase and aldehyde dehydrogenase has evolved from ethanol metabolism to decomposition of aromatic compounds.
Therefore, expression of alcohol dehydrogenase gene and pyruvate decarboxylase gene isolated from baker’s yeast under the control of glyceraldehyde-3-phosphate dehydrogenase promoter from Nameko, are to be investigated to breed ethanol fermentable mushroom

Research Products

• [Journal Article] Differential expression of three α-amylase genes from the basidiomycetous fungus Pholiota microspora (2017)
  • Author(s): Zhu,G., Hayashi,N., Shimomura,N., Yamaguchi, T. and Aimi,T
  • Journal Title: Mycoscience Volume: 58 Issue: 3 Pages: 188-191
  • DOI: 10.1016/j.myc.2017.01.005
  • NAID: 40021207142
  • Peer Reviewed
• [Journal Article] Identification, Characterization and expression of the second glucoamylase gene from Pholiota microspora. (2016)
  • Author(s): 87)Zhu, G., Wan, J., Hayashi, M., Shimomura, N., Yamaguchi, Y., and Aimi, T.
  • Journal Title: Mushroom Science and Biotechnology Volume: 24(2) Pages: 77-84
  • NAID: 130007486901
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Expression of α-glucosidase during morphological differentiation in the basidiomycetous fungus Pholiota microspora (2016)
  • Author(s): Zhu, G., Hayashi, M., Shimomura, N., Yamaguchi, T. and Aimi, T.
  • Journal Title: Journal of Basic Microbiology Volume: 56 Issue: 9 Pages: 1036-1045
  • DOI: 10.1002/jobm.201500752
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Transcriptional analysis of alcohol and aldehyde dehydrogenase gene families in Pholiota microspora, and estimation of their physiological roles. (2016)
  • Author(s): Surasit SUTTHIKHAMPA, Yoshiko KAWAI, Mirai HAYASHI,Sophon BOONLUE, Norihiro SHIMOMURA,Takeshi YAMAGUCHI and Tadanori AIMI
  • Journal Title: Mushroom Science and Biotechnology Volume: Vol. 24(1)
  • NAID: 130007487937
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Expression of genes for the six starch degrading enzyme and their roles for fruiting in the basidiomycetous fungus Pholiota microspora. (2017)
  • Author(s): Itani Y., Zhu, G. Hayashi, M., Shimomura, N., Yamaguchi, T. and Aimi, T.
  • Organizer: The Ubon Ratchathani University Research Conference (UBRC), Ubon Ratchathani, Thailand,
  • Int'l Joint Research
• [Presentation] ナメコ Pholiota microspore におけるデンプン分解酵素遺伝子の発現と子実体形成における役割． (2017)
  • Author(s): 井谷優・朱剛・林未来・霜村典宏・山口武視・會見忠則
  • Organizer: 日本きのこ学会第21回大会，宮崎市．