A study of analysis and utilization of ligninolysis induced by over-expression of a catalytic subunit of protein kinase A.
| Project/Area Number |
15K07517
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Wood science
|
|---|
| Research Institution | The University of Shiga Prefecture |
|---|
Principal Investigator |
IRIE TOSHIKAZU 滋賀県立大学, 環境科学部, 准教授 (30336721)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | リグニン / きのこ / 白色腐朽菌 / バイオマス変換 / ヒラタケ / 品種改良 / リグノセルロース / 窒素代謝 / リグニン分解 / 木質バイオリファイナリー |
|---|
| Outline of Final Research Achievements |
Results of this study collectively suggest that protein kinase A has an import role for expression of ligninolysis in white-rot fungi. Novel selectable maker gene, nourseothricin resistant gene was successfully used for transformation of Pleurotus ostreatus. In addition, the efficiency of P. ostreatus transformation using bialaphos-resistant gene as a selectable maker was improved by our new method. With the aim of construction of custom recombinant strain of P. ostreatus used for conversion of weeds to fish feeds, nitrogen-catabolite repression system of the fungus was analyzed. As the result, it is suggested that P. ostreatus also has a nitrogen-catabolite repression system regulated by AreA (nit2) like as that in ascomycete.
|
Report
(4 results)
Research Products
(10 results)
-
[Journal Article] A development and an improvement of selectable markers in Pleurotus ostreatus transformation.2017
Author(s)
Matsunaga, Y., Ando, M., Izumitsu, K., Suzuki, K., Honda, Y., Irie, T.
-
Journal Title
Journal of Microbiological Methods
Volume: 134
Pages: 27-29
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
[Journal Article] Effects of calmodulin on expressionof lignin-modifyingenzymes in Pleurotus ostreatus2014
Author(s)
Suetomi, T., Sakamoto, T., Tokunaga, Y., Kameyama, T.,Honda, Y., Kamitsuji, H., Kameshita, I., Izumitsu, K., Suzuki, K., Irie, T.
-
Journal Title
Curr. Genet.
Volume: 14
Issue: 2
Pages: 1-14
DOI
Related Report
Peer Reviewed
-
-
-
-
-
