| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Results of this study collectively suggest that protein kinase A has an import role for expression of ligninolysis in white-rot fungi. Novel selectable maker gene, nourseothricin resistant gene was successfully used for transformation of Pleurotus ostreatus. In addition, the efficiency of P. ostreatus transformation using bialaphos-resistant gene as a selectable maker was improved by our new method. With the aim of construction of custom recombinant strain of P. ostreatus used for conversion of weeds to fish feeds, nitrogen-catabolite repression system of the fungus was analyzed. As the result, it is suggested that P. ostreatus also has a nitrogen-catabolite repression system regulated by AreA (nit2) like as that in ascomycete.
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