| Project/Area Number |
15K07552
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Aquatic bioproduction science
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| Research Institution | Nagasaki University |
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Principal Investigator |
SUGA Koushirou 長崎大学, 水産・環境科学総合研究科(水産), 准教授 (60569185)
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| Co-Investigator(Renkei-kenkyūsha) |
KANAI Kinya 長崎大学, 水産・環境科学総合研究科(水産), 教授 (40145222)
SAKAKURA Yoshitaka 長崎大学, 水産・環境科学総合研究科(水産), 教授 (20325682)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 魚病細菌 / 抗病性 / 仔魚飼育 / 健苗性 |
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| Outline of Final Research Achievements |
In this study, attempted to confer resistance to bacterial pathogens on fish by feeding bacteria to fish larva. Quantitative PCR (qPCR) was applied for counting the number of bacteria.
In order to investigate the effect of different DNA purification kits in counting bacterial cells using qPCR, we compared two methods; the bacterial culture dilution method, in which the DNA is purified after 5 steps of a ten-fold serial dilution of bacterial culture, and the DNA dilution method, which the DNA is diluted after purification with the optimum cell number of bacteria. In both methods, we used three types of DNA purification kit. Our results showed the lowest Ct value and high DNA recovery rate were obtained using Chelex resin. The survival rate of the fish larva, which fed the zooplankton containing bacteria, was equivalent to that of the control. Tissue section analysis revealed that there was no difference the bacteria-fed fish larva and control.
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