| Project/Area Number |
15K07555
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Aquatic bioproduction science
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
酒井 正博 宮崎大学, 農学部, 教授 (20178536)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | クルマエビ / 生体防御因子 / ホルモン / ＷＳＳＶ / ウイルス / 細菌 / サイトカイン / インスリン様ホルモン / 低酸素 |
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| Outline of Final Research Achievements |
In shrimp aquaculture, high-stocking farming with phytoplankton blooming decreases the dissolved oxygen concentrations in the pond water. Exposure of shrimp to hypoxia induces the molting and molted shrimp are susceptible to infection due to its physical and physiological weakness, e.g. soft shell and loss of energy.
Hypoxia-inducible factor (HIF) is a transcriptional factor in the basic helix-loop-helix/PAS family and is activated in response to hypoxic stress. However, information of HIF and other related factors of the HIF pathway in crustaceans are not well known. In this study, we cloned MjHIF-1α, an inhibitory factor, MjFIH-1, and MjVHL from kuruma shrimp (Marsupenaeus japonicus). MjVHL is the first crustacean VHL ortholog cloned in this study. MjHIF-1α and MjVHL were highly expressed in the intestine. MjHIF-1α expression in the hypoxic group increased significantly 24 h after the hypoxia exposure and expression of MjVHL decreased significantly 6 h after hypoxia stimulation.
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