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Analysis of gene structure to determine serotype of Flavobacterium psychrophilum and its application in the development of epidemiological marker

Research Project

Project/Area Number 15K07561
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Aquatic bioproduction science
Research InstitutionTokai University

Principal Investigator

Izumi Shotaro  東海大学, 海洋学部, 准教授 (90450379)

Co-Investigator(Kenkyū-buntansha) 新井 肇  群馬県水産試験場, その他部局等, 研究員 (60450384)
Research Collaborator SUZUKI Kyuma  群馬県水産試験場, 研究員 (80450386)
WATANABE Syun  群馬県水産試験場, 研究員 (30739024)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords冷水病 / 血清型 / 疫学調査 / PCR-RFLP
Outline of Final Research Achievements

In order to develop a simple and inexpensive epidemiological marker by serotyping for bacterial cold-water disease, DNA sequence mutations and genetic structure involved in serotype determination of Flavobacterium psychrophilum were analyzed. Several mutations were confirmed, but nothing characteristic for each serotype was found. Unfortunately, it was impossible to achieve the expected results in the technical improvement of serotyping and the confirmation of host specificity, in this research period.
In the epidemiological survey in the river where bacterial cold-water disease outbreaking, 357 strains of F. psychrophilum were successfully isolated and typed by the previously reported genotypings. As a result, bacterial cold-water diseases in this river consisted of multiple sources of infection and the existence of highly frequent genotype was found. We were able to gain the required achievement as an epidemiological survey.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report

Research Products

(4 results)

All 2018 2016 Other

All Journal Article Presentation Remarks

  • [Journal Article] Identification and expression analysis of IRAK-4 cDNA and its gene from the ayu Plecoglossus altivelis altivelis2016

    • Author(s)
      K. Suzuki, S. Izumi, H. Tanaka, and T. Katagiri
    • Journal Title

      Fisheries Science

      Volume: 82 (1) Pages: 47-57

    • DOI

      10.1007/s12562-015-0942-z

    • NAID

      40020718555

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] 神流川で発生したアユ冷水病の流行型2018

    • Author(s)
      新井肇・渡辺峻・鈴木究真・泉庄太郎
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 神流川におけるアユ冷水病の伝播経路の解明2016

    • Author(s)
      新井肇・渡辺峻・湯浅由美・鈴木究真・田中英樹・久下敏宏・泉庄太郎
    • Organizer
      平成28年度日本水産学会秋季大会
    • Place of Presentation
      近畿大学農学部(奈良キャンパス)
    • Related Report
      2016 Research-status Report
  • [Remarks] 旬の話題「神流川で冷水病の伝播経路を調査しています」

    • URL

      http://www.pref.gunma.jp/07/p15200171.html

    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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