| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The aim of this study was to assess whether infectious piscine pathogenic viruses such as viral hemorrhagic septicemia virus (VHSV), Cyprinid herpesvirus-2 (CyHV-2) and Cyprinid herpesvirus-3 (CyHV-3) can remain viable in crustacean zooplankton and can be transferred to and remain viable in a fish host. Since water fleas are cultivable under laboratory condition, water flea (Moina macrocopa) was adopted as a model crustacean zooplankton. This study demonstrated that VHSV can be taken up from the environmental water by water fleas and can remain viable within water fleas for several days. CyHV-2 and CyHV-3 do however not isolate from the water fleas. No mortality was observed in rainbow trout, goldfish and carp which were fed the water fleas contaminated with piscine pathogenic viruses during 14 days. Thus, it is unlikely that this crustacean act as a mechanical vector of VHSV, CyHV-2 and CyHV-3 to rainbow trout, goldfish and carp, respectively.
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