The endocrine system controlling crustacean reproduction: roles of ovarian hormonal factors

• Project/Area Number: 15K07576
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Okayama University
• Principal Investigator: Naoaki Tsutsui 岡山大学, 理学部, 助教 (00643785)
• Co-Investigator(Renkei-kenkyūsha): OKUMURA TAKUJI 国立研究開発法人水産研究・教育機構, 増養殖研究所, 主幹研究員 (30372030)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 甲殻類 / 生殖 / 卵黄形成 / ホルモン / 増養殖 / クルマエビ / 内分泌

Outline of Final Research Achievements

Crustacean reproduction is principally regulated by vitellogenesis- inhibiting hormone from the X-organ/sinus gland neurosecretory system in the eyestalks. On the other hand, the roles of peptide/protein hormones that expressed in peripheral tissues are still unclear. In this study, we focused on hormonal factors of which transcripts were found by the transcriptome analysis of kuruma prawn ovary. Gene expression analysis, histological analysis, and functional analysis using recombinant protein suggested that a few arthropod hormone homologs may be involved in the regulation of reproductive process in the kuruma prawn.

Research Products

• [Journal Article] Characterization of distinct ovarian isoform of crustacean female sex hormone in the kuruma prawn Marsupenaeus japonicus (2018)
  • Author(s): Naoaki Tsutsui, Sayaka Kotaka, Tsuyoshi Ohira, Tatsuya Sakamoto
  • Journal Title: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology Volume: 217 Pages: 7-16
  • DOI: 10.1016/j.cbpa.2017.12.009
  • Peer Reviewed
• [Journal Article] Involvement of second messengers in the signaling pathway of vitellogenesis-inhibiting hormone and their effects on vitellogenin mRNA expression in the whiteleg shrimp, Litopenaeus vannamei (2017)
  • Author(s): Bae, S-H., Okutsu, T., Tsutsui, N., Kang, B.J., Chen, H-Y., Wilder, M.N.
  • Journal Title: General and Comparative Endocrinology Volume: 246 Pages: 301-308
  • DOI: 10.1016/j.ygcen.2017.01.006
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Crystal structure of a crustacean hyperglycemic hormone (CHH) precursor suggests structural variety in the C-terminal regions of CHH superfamily members. (2016)
  • Author(s): Tsutsui N, Sakamoto T, Arisaka F, Tanokura M, Nagasawa H, Nagata K
  • Journal Title: FEBS J Volume: 283 Issue: 23 Pages: 4325-4339
  • DOI: 10.1111/febs.13926
  • Peer Reviewed / Open Access
• [Journal Article] Artificial burrow sizes for capturing the North American invasive crayfish <i>Procambarus clarkii</i>. (2015)
  • Author(s): Ushimi H., Miyatake Y., Tsutsui N., Sakamoto T., Nakata K.
  • Journal Title: Ecology and Civil Engineering Volume: 18 Issue: 2 Pages: 79-86
  • DOI: 10.3825/ece.18.79
  • NAID: 130005122067
  • ISSN: 1344-3755, 1882-5974
  • Peer Reviewed
• [Presentation] The search for hormone-like transcripts expressed in the prawn ovary using next generation sequencing. (2017)
  • Author(s): Naoaki Tsutsui, Sayaka Kotaka, Tsuyoshi Ohira, Yasuhisa Kobayashi, Tatsuya Sakamoto.
  • Organizer: 14th International Congress on Invertebrate Reproduction and Development
  • Int'l Joint Research
• [Presentation] Isolation and cDNA cloning of molt-inhibiting hormone (MIH) from the southern rough shrimp Trachysalambria curvirostris. (2017)
  • Author(s): Masafumi Hanazuka, Naoaki Tsutsui, Tsuyoshi Ohira.
  • Organizer: The Crustacean Society Mid-Year Meeting
  • Int'l Joint Research
• [Presentation] クルマエビの卵巣で発現するペプチドホルモン様因子. (2017)
  • Author(s): 筒井直昭, 坂本竜哉.
  • Organizer: 第42回日本比較内分泌学会大会
• [Presentation] クルマエビ甲殻類雌性ホルモンの周年変動 (2017)
  • Author(s): 甲高彩華, 筒井直昭, 大平剛
  • Organizer: 第42回日本比較内分泌学会大会
• [Presentation] Expression of a recombinant vitellogenesis-inhibiting hormone of the southern rough shrimp Trachysalambria curvirostris in Escherichia coli. (2016)
  • Author(s): Hanazuka M., Kaji K., Yoshida Y., Tsutsui N., Ohira T.
  • Organizer: The 87th Meeting of Zoological Society of Japan
  • Place of Presentation: 沖縄
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Localization of two crustacean female sex hormones in the kuruma prawn Marsupenaeus japonicus (2016)
  • Author(s): Kotaka S., Tsutsui N., Ohira T.
  • Organizer: 28th Conference of European Comparative Endocrinologists
  • Place of Presentation: ベルギー
  • Year and Date: 2016-08-21
  • Int'l Joint Research