| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We compared the fish-killing activities of Chattonella marina isolated in 1985 and Chattonella antiqua isolated in 2010. Nearly 100% of the fish species died within a few hours exposure to C. antiqua at 10,000 cells/ml, whereas no dead fish were observed even 24 h after initial exposure to C. marina at 10,000 cells/ml. Chemiluminescence and electron spin resonance (ESR) analyses suggested greater reactive oxygen species (ROS)-producing activity of C. antiqua than of C. marina. These results suggest that the ROS-producing activity and affinity of Chattonella cells to the gill surface may be important factors for the fish-killing activity. We also investigated the effects of two strains of the dinoflagellate Karenia mikimotoi (SUO-1 and FUK) on the rotifer. The strain SUO-1 showed highly toxic effect on rotifer, whereas the strain FUK showed obviously lower toxicity. The cell suspension of SUO-1 showed a hemolytic activity toward horse erythrocytes, but FUK did not.
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