Does pufferfish intake tetrodotoxin by brain reward system?

• Project/Area Number: 15K07581
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Nagasaki University
• Principal Investigator: SAKAKURA Yoshitaka 長崎大学, 水産・環境科学総合研究科(水産), 教授 (20325682)
• Co-Investigator(Kenkyū-buntansha): 天野 勝文 北里大学, 海洋生命科学部, 教授 (10296428)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: フグ毒 / トラフグ / 食欲 / 成長 / 遺伝子発現 / 水産学 / 生理 / フグ

Outline of Final Research Achievements

We hypothesized that tetrodotoxin (TTX) sensing will induce appetite and administration of TTX by food will induce growth in the non-toxic artificially-raised tiger puffer juveniles. After fish were immersed in the TTX solution, gene expression of Neuro-peptide Y (NPY) was quantified by RT-PCR. Higher trend of NPY genes expression in the brain of TTX-sensed fish was detected. When fish were fed with TTX-containing diet for 35 days, fish showed significantly higher growth than control diet. These results supported our hypothesis.

Research Products

• [Journal Article] Administration of tetrodotoxin protects artificially-raised juvenile tiger puffer Takifugu rubripes from predators. (2017)
  • Author(s): Sakakura, Y., Takatani, T., Nakayasu, J., Yamazaki, H., Sakiyama, K.
  • Journal Title: Fisheries Science Volume: 83 Issue: 2 Pages: 191-198
  • DOI: 10.1007/s12562-016-1046-0
  • NAID: 120006987423
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Transcriptome analysis of tetrodotoxin sensing and action of tetrodotoxin in central nervous system of tiger puffer Takifugu rubripes juveniles (2017)
  • Author(s): Okita, K., Tan, E., Satone, H., Kinoshita, S., Asakawa, S., Ojima, D., Yamazaki, H., Sakiyama, K., Takatani, T., Arakawa, O., Hagiwara, A., Sakakura, Y.
  • Journal Title: Fisheries Science Volume: 83 Issue: 3 Pages: 401-412
  • DOI: 10.1007/s12562-017-1079-z
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] トラフグ人工種苗の成長に伴う行動と食性の変化 (2015)
  • Author(s): 山根 晃・吉田 歩・山崎英樹・崎山一孝・河端雄毅・阪倉良孝
  • Journal Title: 水産増殖 Volume: 63 Pages: 141-149
  • NAID: 130005157558
  • Peer Reviewed
• [Presentation] Seeking the effective method for release of hatchery-reared tiger puffer juvenile. (2017)
  • Author(s): Sakakura Yoshitaka
  • Organizer: The 11th International Workshop on the Oceanography and Fisheries Science of the East China Sea
• [Presentation] フグ毒添加飼料がトラフグ稚魚の成長に与える影響 (2017)
  • Author(s): 阪倉良孝、佐藤はるか、天野勝文、高谷智裕
  • Organizer: 平成30年度日本水産学会春季大会
• [Presentation] Evaluation of fish quality and microhabitat for release in the hatchery-reared tiger puffer juveniles (2015)
  • Author(s): Sakakura, Y. Takatani, T., Yamane, H., Nakayasu, J., Yamazaki, H., Sakiyama, K.
  • Organizer: 5th International Symposium on Stock Enhancement and Sea Ranching
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2015-10-11
  • Int'l Joint Research
• [Presentation] Transcriptome analysis of tetrodotoxin sensing and action of tetrodotoxin in the brain of tiger puffer Takifugu rubripes by next-generation sequencing (2015)
  • Author(s): Okita, K., Satone, H., Tan, E., Kinoshita, S., Asakawa, S., Yamazaki, H., Sakiyama, K., Takatani, T., Arakawa, O., Hagiwara, A., Sakakura, Y.
  • Organizer: American Fisheries Society Annual Meeting
  • Place of Presentation: Portland, U.S.A.
  • Year and Date: 2015-08-14
  • Int'l Joint Research
• [Presentation] Factors affecting the fear response of hatchery-reared tiger puffer Takifugu rubripes juveniles (2015)
  • Author(s): Sakakura, Y., Takatani, T., Yamane, H., Nakayasu, J., Okita, K., Yoshida, A., Yamazaki, H., Sakiyama, K.
  • Organizer: American Fisheries Society Annual Meeting
  • Place of Presentation: Portland, U.S.A.
  • Year and Date: 2015-08-14
  • Int'l Joint Research
• [Remarks] 水産・環境科学総合研究科の阪倉良孝教授の研究グループが平成29年度日本水産学会論文賞を受賞
  • URL: http://www.nagasaki-u.ac.jp/ja/about/info/news/news2611.html
• [Remarks] 阪倉長崎大教授 人工種苗フグ毒化で生残向上
  • URL: http://www.minato-yamaguchi.co.jp/minato/week1/2016/jun/m160615.html