Screening novel peptide toxins of sea anemones by new assay systems
Project/Area Number |
15K07592
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Aquatic life science
|
Research Institution | Tokai University Junior College |
Principal Investigator |
HONMA TOMOHIRO 東海大学短期大学部, 東海大学短期大学部, 准教授 (90435272)
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | イソギンチャク / ペプチド毒 / イオンチャネル / Biacore / 膜電位固定法 / サワガニ / cDNAクローニング / Biacore |
Outline of Final Research Achievements |
The assay systems were investigated for screening novel peptide toxins of sea anemone. The investigation of the ion channel-binding assay system with the use of surface plasmon resonance system (Biacore) was the new attempt. However, its utilization proved to be unable due to the difficulty of reducing nonspecific binding between its toxin and rat brain synaptosomes. On the other hand, the novel peptide toxins succeeded in being isolated from various sea anemones by investigating the assay system with the use of freshwater crabs. Furthermore, the mode of action of the novel peptide toxins which have been ever isolated succeeded in being clarified by the two-electrode voltage-clamp (TEVC).
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Report
(4 results)
Research Products
(3 results)