| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Exosomes are extracellular small granule vesicles produced by cells, and might mediate a signal transduction mechanisms to transfer the information between cells and organisms. They contain various molecular constituents, such as, proteins, DNA, mRNA and miRNA, and transfer molecules from cells through membrane vesicle trafficking. We found that exosomes were secreted by methylmercury (MeHg) treatment in zebrafish embryos,and the exosomes had protective functions. To visualize the dynamics of exosomes in vivo, cd63-GFP expressing zebrafish lines were used. Proteomic analysis of exosomes revealed that MeHg induced proteins were identified,and sensitivity to MeHg was associated with the presence of these proteins. These results indicated that exosome-mediated inter-individual communication played an important role under stress conditions.
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