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Verification of biooxidation of ciguatoxins leading to species specific toxin profiles

Research Project

Project/Area Number 15K07594
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Aquatic life science
Research InstitutionFisheries Research and Education Agency (2016-2017)
National Fisheries University (2015)

Principal Investigator

Ikehara Tsuyoshi  国立研究開発法人水産研究・教育機構, 水産大学校, 教授 (90359951)

Co-Investigator(Renkei-kenkyūsha) OSHIRO Naomasa  国立医薬品食品衛生研究所, 食品衛生管理部第二室, 室長 (20507032)
Research Collaborator YASUMOTO Takeshi  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsシガテラ / シガトキシン / 酸化酵素 / 海洋自然毒
Outline of Final Research Achievements

Ciguatera fish poisoning is a clinical syndrome that causes neurological symptoms in patients and affects more than 50,000 people per year. This foodborne illness results from eating fish from warm water regions around the world that contain natural marine toxins that are collectively known as ciguatoxins (CTXs). In the Pacific, these toxins are produced by the dinoflagellate Gambierdiscus toxicus, which accumulates in fish through the food chain and undergoes oxidative modification, giving rise to numerous analogs. In this study, we aimed to verify the biooxidation process of CTXs in vitro using CTX4A/4B and CTX3C as substrates, and recombinant human CYP3A4, fish liver S9 fractions, and microsomal fractions as oxidizing enzymes. The reaction products were identified by LC-MS/MS analysis using reference toxins, which unambiguously confirmed the production of CTX1B, 54-deoxyCTX4B, 52-epi-54-deoxyCTX1B, 2-hydroxyCTX3C, and 2,3-dihydroxyCTX3C.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (12 results)

All 2018 2017 2016 2015

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (6 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Biooxidation of Ciguatoxins Leads to Species-Specific Toxin Profiles2017

    • Author(s)
      Ikehara Tsuyoshi、Kuniyoshi Kyoko、Oshiro Naomasa、Yasumoto Takeshi
    • Journal Title

      Toxins

      Volume: 9 Issue: 7 Pages: 205-205

    • DOI

      10.3390/toxins9070205

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] タンパク質脱リン酸化酵素2A(PP2A)を利用した下痢性貝毒簡易検査法の評価2017

    • Author(s)
      池原強,木下翼,黒川純花,中島志穂子,前川公彦,大城直雅,安元健
    • Journal Title

      日本水産学会誌

      Volume: 83 Pages: 367-372

    • NAID

      130007331243

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient production of recombinant PP2A at a low temperature using a baculovirus expression system.2016

    • Author(s)
      Tsuyoshi Ikehara,Shihoko Nakashima,Junichi Nakashima,Tsubasa Kinoshita,Takeshi Yasumoto
    • Journal Title

      Biotechnology Reports

      Volume: Vol.11 Pages: 86-89

    • DOI

      10.1016/j.btre.2016.07.004

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Different responses of primary normal human hepatocytes and human hepatoma cells toward cyanobacterial hepatotoxin microcystin-LR2015

    • Author(s)
      Tsuyoshi Ikehara, Junichi Nakashima, Shihoko Nakashima, Takeshi Yasumoto
    • Journal Title

      Toxicon

      Volume: 105 Pages: 4-9

    • DOI

      10.1016/j.toxicon.2015.08.025

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Enhancer of Acetyltransferase Chameau(EAChm) Is a Novel Transcriptional Co-Activator2015

    • Author(s)
      Takeya Nakagawa, Tsuyoshi Ikehara, Masamichi Doiguchi, Yuko Imamura, Miki Higashi, Mitsuhiro Yoneda, Takashi Ito
    • Journal Title

      PLoS ONE

      Volume: 10(11) Issue: 11 Pages: e0142305-e0142305

    • DOI

      10.1371/journal.pone.0142305

    • NAID

      120006987224

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 下痢性貝毒の簡易検査法の開発‐国際基準の導入へ向けた取り組み‐2015

    • Author(s)
      池原 強
    • Journal Title

      海洋水産エンジニアリング

      Volume: 122 Pages: 117-121

    • NAID

      40020536314

    • Related Report
      2015 Research-status Report
  • [Presentation] 酸化酵素によるシガトキシンの化学構造修飾2018

    • Author(s)
      池原強
    • Organizer
      シガトキシン関連資料展示記念ワークショップ
    • Related Report
      2017 Annual Research Report
  • [Presentation] ホタテガイ(Patinopecten yessoensis)可食部試料を用いたPP2A活性阻害法による下痢性貝毒簡易分析法の評価2016

    • Author(s)
      木下翼,池原強,前川公彦,中島志穂子,大城直雅,安元健
    • Organizer
      平成28年度日本水産学会秋季大会
    • Place of Presentation
      近畿大学農学部奈良キャンパス
    • Year and Date
      2016-09-08
    • Related Report
      2016 Research-status Report
  • [Presentation] Verification of biooxidation products of ciguatoxins leading to species specific toxin profiles2016

    • Author(s)
      Tsuyossi Ikehara
    • Organizer
      First Workshop on Emerging Toxins
    • Place of Presentation
      Spain
    • Year and Date
      2016-09-05
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 酸化酵素によるシガトキシンの化学構造修飾2016

    • Author(s)
      池原 強,大城直雅,國吉杏子,安元健
    • Organizer
      第30 回海洋生物活性談話会
    • Place of Presentation
      鳥取大学
    • Year and Date
      2016-05-14
    • Related Report
      2016 Research-status Report
  • [Presentation] ヒト正常肝細胞(h-Nheps) およびヒト肝がん由来細胞(HepG2)に対するミクロシスチン-LRの毒性評価2015

    • Author(s)
      池原 強,中島 淳一,中島 志穂子,安元 健
    • Organizer
      第38回日本分子生物学会年会,第88回日本生化学会大会 合同大会
    • Place of Presentation
      兵庫県
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] シガテラ魚CTX酸化酵素の検出2015

    • Author(s)
      池原 強,大城直雅,安元健
    • Organizer
      第29回海洋生物活性談話会
    • Place of Presentation
      静岡県
    • Year and Date
      2015-05-09
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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