Treatment of mastitis using temporal cessation of milking

• Project/Area Number: 15K07692
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal production science
• Research Institution: Hiroshima University
• Principal Investigator: Isobe Naoki 広島大学, 生物圏科学研究科, 准教授 (80284230)
• Co-Investigator(Renkei-kenkyūsha): OKITA MIKI 広島大学, 大学院生物圏科学研究科, 助教 (30611842)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 乳房炎 / エストラジオール / 抗菌因子 / ショート乾乳 / 乳量 / エストロゲン / 水チャネル

Outline of Final Research Achievements

The aim was to explore the effect of estrogen and temporal cessation of milking for 3 days on mammary immune function in order to develop new mastitis treatment system. Estrogen injection or temporal cessation of milking reduced milk yield and increased antimicrobial components concentration in milk. Combination of E treatment and temporal cessation of milking upregulated antimicrobial components in milk dramatically compared with that only temporal cessation of milking. These results suggest that temporal cessation of milking together with E augmented innate immune function which can be utilized to the treatment of mastitis.

Research Products

• [Journal Article] Detection of the APAF1 mutation in Holstein cows and mummified fetuses in Japanese dairy herds. (2018)
  • Author(s): Ghanem M, Nishibori M, Isobe N, Hisaeda, K
  • Journal Title: Reproduction in Domestic Animals Volume: 53 Issue: 1 Pages: 137-142
  • DOI: 10.1111/rda.13081
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Blood ionized calcium levels and acute-phase blood glucose kinetics in goats after intramammary infusion of lipopolysaccharide (2018)
  • Author(s): Shinozuka Y, Kawai K, Sato R, Higashitani A, Hamamoto Y, Okita M, Isobe N
  • Journal Title: Journal of Veterinary Medical Science Volume: 80 Issue: 2 Pages: 242-246
  • DOI: 10.1292/jvms.17-0615
  • NAID: 130006339635
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Microcystin-leucine-arginine Modulates the Expression Patterns of Proinflammatory Cytokines and an Apoptotic Gene in Chicken Liver (2018)
  • Author(s): Elgawish RA, Yoshimura Y, Isobe N
  • Journal Title: The Journal of Poultry Science Volume: 55 Issue: 1 Pages: 70-77
  • DOI: 10.2141/jpsa.0170054
  • NAID: 130006320879
  • ISSN: 1346-7395, 1349-0486
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Changes in plasma concentrations of S100A7 and S100A8 in dairy cows during pregnancy. (2018)
  • Author(s): Elgawish RA, Ogata Y, Hidaka T, Nii T, Yoshimura Y, Isobe N
  • Journal Title: Reproduction in Domestic Animals Volume: -
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Effects of colostrum whey on immune function in the digestive tract of goats. (2018)
  • Author(s): Matsukawa S, Ueno K, Sugino T, Yoshimura Y, Isobe N
  • Journal Title: Animal Science Journal Volume: -
  • Peer Reviewed
• [Journal Article] Changes in the concentrations of somatic cell counts, lingual antimicrobial peptide and lactoperoxidase activity in milk at periovulatory period in dairy cows. (2017)
  • Author(s): Yamasaki A, Yoshimura Y, Isobe N
  • Journal Title: Animal Science Journal Volume: 88 Issue: 3 Pages: 484-488
  • DOI: 10.1111/asj.12644
  • Peer Reviewed
• [Journal Article] Control mechanisms for producing antimicrobial factors in ruminant mammary gland. (2017)
  • Author(s): Isobe N
  • Journal Title: Animal Science Journal Volume: 88 Issue: 7 Pages: 937-943
  • DOI: 10.1111/asj.12808
  • Peer Reviewed
• [Journal Article] Effect of steroid hormones on the innate immune response induced by Staphylococcus aureus in the goat mammary gland. (2017)
  • Author(s): Kuwahara K, Yoshimura Y, Isobe N
  • Journal Title: Reproduction in Domestic Animals Volume: 52 Issue: 4 Pages: 579-584
  • DOI: 10.1111/rda.12948
  • Peer Reviewed
• [Journal Article] Cellular and soluble components decrease the viable pathogen counts in milk from dairy cows with subclinical mastitis (2017)
  • Author(s): Koshiishi T, Watanabe M, Miyake H, Hisaeda K, Isobe N
  • Journal Title: Journal of Veterinary Medical Science Volume: 79 Issue: 8 Pages: 1389-1393
  • DOI: 10.1292/jvms.17-0269
  • NAID: 130005950127
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] The inhibitory effect of sodium thiocyanate and sodium percarbonate rations on microorganism growth in raw milk samples as an effective treatment to extend milk quality during storage. (2017)
  • Author(s): Srisaikham S, Isobe N, Suksombat W
  • Journal Title: Songklanakarin Journal of Science and Technology Volume: 39 Pages: 77-89
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Goat cathelicidin-2 is secreted by blood leukocytes regardless of lipopolysaccharide stimulation. (2016)
  • Author(s): Srisaikham S, Suksombat W, Yoshimura Y, Isobe N
  • Journal Title: Animal Science Journal Volume: 87 Issue: 3 Pages: 423-427
  • DOI: 10.1111/asj.12438
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Change in viable bacterial count during preservation of milk derived from dairy cows with subclinical mastitis and its relationship with antimicrobial components in milk (2016)
  • Author(s): Hisaeda K, Koshiishi T, Watanabe M, Miyake H, Yoshimura Y, Isobe N
  • Journal Title: Journal of Veterinary Medical Science Volume: 78 Issue: 8 Pages: 1245-1250
  • DOI: 10.1292/jvms.16-0049
  • NAID: 130005261515
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Changes in the concentrations of somatic cell counts, lingual antimicrobial peptide and lactoperoxidase activity in milk at periovulatory period in dairy cows. (2016)
  • Author(s): Yamasaki A, Yoshimura Y, Isobe N
  • Journal Title: Animal Science Journal Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 乳房炎Q&A「なぜ乳房炎が繁殖機能に影響を及ぼすのか」 (2016)
  • Author(s): 磯部直樹・久枝啓一
  • Journal Title: 臨床獣医 Volume: 34 Pages: 4-5
• [Journal Article] Dynamics of lingual antimicrobial peptide, lactoferrin concentrations and lactoperoxidase activity in the milk of cows treated for clinical mastitis. (2015)
  • Author(s): Kawai K, Korematsu K, Akiyama K, Okita M, Yoshimura Y, Isobe N
  • Journal Title: Animal Science Journal Volume: 86 Issue: 2 Pages: 153-158
  • DOI: 10.1111/asj.12269
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Changes in S100A8 concentration in milk after intra-mammary infusion of lipopolysaccharide. (2018)
  • Author(s): Fika Yuliza Purba, Takahiro Nii, Yukinori Yoshimura, Naoki Isobe
  • Organizer: 日本畜産学会第124回大会
• [Presentation] 初乳製剤を用いたウシの健康管理 (2018)
  • Author(s): 磯部直樹
  • Organizer: 第41回NOSAI診療技術研究発表会
  • Invited
• [Presentation] ヤギ乳腺上皮細胞におけるカテリシジン-7の遺伝子発現に及ぼす微生物刺激の影響 (2017)
  • Author(s): 西川萌美・吉村幸則・磯部直樹
  • Organizer: 日本畜産学会第122回大会
  • Place of Presentation: 神戸大学
  • Year and Date: 2017-03-28
• [Presentation] 3日間の搾乳停止が乳腺自然免疫機能へ及ぼす影響． (2017)
  • Author(s): 磯部直樹・植田丈・吉村幸則
  • Organizer: 日本畜産学会第123回大会
• [Presentation] ヤギにおける妊娠中期の乳房炎が妊娠機能に及ぼす影響． (2017)
  • Author(s): 兒玉望・新居隆浩・吉村幸則・磯部直樹
  • Organizer: 平成29年度第67回関西畜産学会大会
• [Presentation] スコアⅠ臨床型乳房炎を発症した原因菌発育陰性牛への対応． (2017)
  • Author(s): 佐藤太郎・後藤 洋・三浦道三郎・近藤寧子・赤松裕久・田中秀和・藤田宏子・渡邉あい子・三好志朗・宮田雅章・荒木香里・磯部直樹・篠塚康典・河合一洋
  • Organizer: 第22回日本乳房炎研究会学術集会
• [Presentation] 乳牛の潜在性乳房炎におけるセラメーラの効果． (2017)
  • Author(s): 久枝啓一・輿石智子・北林雅夫・山本周平・磯部直樹
  • Organizer: 第22回日本乳房炎研究会学術集会
• [Presentation] 乳牛の前絞り乳における抗菌因子の濃度変化． (2017)
  • Author(s): 磯部直樹・北野菜奈・髙橋俊彦・吉村幸則
  • Organizer: 第22回日本乳房炎研究会学術集会
• [Presentation] ショート乾乳と抗菌因子 (2017)
  • Author(s): 磯部直樹
  • Organizer: Bovine Mastitis Research (BMR)
  • Invited
• [Presentation] 乳房炎と自然免疫機構 (2017)
  • Author(s): 磯部直樹
  • Organizer: 第21回大動物臨床教育セミナー
  • Invited
• [Presentation] 乳房炎防除のための自然免疫機能の利用 (2017)
  • Author(s): 磯部直樹
  • Organizer: 第22回日本乳房炎研究会学術集会
  • Invited
• [Presentation] 乳腺の抗菌因子と乳房炎防除管理 (2017)
  • Author(s): 磯部直樹
  • Organizer: 第3回広島大学酪農技術セミナー
  • Invited
• [Presentation] 乳房炎防除のための自然免疫機能の利用 (2017)
  • Author(s): 磯部直樹
  • Organizer: 中四国乳房炎協議会第22回勉強会
  • Invited
• [Presentation] 乳房炎の防除管理について (2017)
  • Author(s): 磯部直樹
  • Organizer: 酪農経営支援研修会
  • Invited
• [Presentation] 乳房炎乳保存中における生菌数の変化とその原因 (2017)
  • Author(s): 磯部直樹
  • Organizer: 第40回NOSAI診療技術研究発表会
  • Place of Presentation: NOSAI広島 家畜臨床研修所東広島家畜診療所
  • Invited
• [Presentation] 乳房炎と自然免疫機構 (2017)
  • Author(s): 磯部直樹
  • Organizer: 大動物臨床研究会第7回東京シンポジウム
  • Place of Presentation: 東京大学
  • Invited
• [Presentation] ルーメンアシドーシスによる乳房炎について (2017)
  • Author(s): 磯部直樹
  • Organizer: ルーメン研究会
  • Place of Presentation: 神戸大学
  • Invited
• [Presentation] 乳房炎と自然免疫機構 (2016)
  • Author(s): 磯部直樹
  • Organizer: 第40回大動物臨床研究会
  • Place of Presentation: 酪農学園大学
  • Year and Date: 2016-11-05
  • Invited
• [Presentation] 初乳乳清の経口投与がヤギ消化管の免疫機能に及ぼす影響 (2016)
  • Author(s): 松川 祥子・上野 和俊・杉野 利久・磯部 直樹・吉村 幸則:
  • Organizer: 平成28年度第66回関西畜産学会大会
  • Place of Presentation: 香川大学
  • Year and Date: 2016-10-15
• [Presentation] 黄色ブドウ球菌により誘起されたヤギ乳腺の自然免疫応答に及ぼす性ステロイドホルモンの影響 (2016)
  • Author(s): 磯部直樹・桑原謙太・吉村幸則
  • Organizer: 第109回日本繁殖生物学会大会
  • Place of Presentation: 麻布大学
  • Year and Date: 2016-09-12
• [Presentation] 乳房炎が妊娠ヤギの内分泌機能に及ぼす影響 (2016)
  • Author(s): 兒玉望・吉村幸則・磯部直樹
  • Organizer: 第3回乳房炎サマーキャンプ
  • Place of Presentation: 山梨県吉野荘
  • Year and Date: 2016-09-03
• [Presentation] Effect of steroid hormone on innate immune response induced by Staphylococcus aureus in mammary gland of goat． (2016)
  • Author(s): Isobe N, Kuwahara K, Yoshimura Y
  • Organizer: The 17th AAAP Animal Science Congress
  • Place of Presentation: Fukuoka
  • Year and Date: 2016-08-22
  • Int'l Joint Research
• [Presentation] Effect of oral administration of colostrum on immune function in the goat digestive tract． (2016)
  • Author(s): Matsukawa S, Sugino T, Yoshimura Y, Isobe N
  • Organizer: The 17th AAAP Animal Science Congress
  • Place of Presentation: Fukuoka
  • Year and Date: 2016-08-22
  • Int'l Joint Research
• [Presentation] Effect of mastitis on pregnancy function in prepartum goats． (2016)
  • Author(s): Kodama N, Yoshimura Y, Isobe N
  • Organizer: The 17th AAAP Animal Science Congress
  • Place of Presentation: Fukuoka
  • Year and Date: 2016-08-22
  • Int'l Joint Research
• [Presentation] Effect of temporary cessation of milking on antimicrobial components in goat milk． (2016)
  • Author(s): Ueda J, Yoshimura Y, Isobe N
  • Organizer: The 17th AAAP Animal Science Congress
  • Place of Presentation: Fukuoka
  • Year and Date: 2016-08-22
  • Int'l Joint Research
• [Presentation] ヤギ白血球におけるカテリシジン-7の発現とそのリポ多糖に対する作用． (2016)
  • Author(s): 西川萌美・吉村幸則・磯部直樹
  • Organizer: 日本畜産学会第121回大会
  • Place of Presentation: 日本獣医生命科学大学（東京都・武蔵野市）
  • Year and Date: 2016-03-28
• [Presentation] ヤギ乳腺におけるカテリシジンー７の発現・分泌とその乳房炎との関係 (2016)
  • Author(s): 西川萌美・吉村幸則・磯部直樹
  • Organizer: 第21回日本乳房炎研究会学術集会
  • Place of Presentation: 国立科学博物館
• [Presentation] Changes in pathogen number during preservation of milk derived from mastitic dairy cows. (2015)
  • Author(s): Isobe N, Hisaeda K, Koshiishi T, Watanabe M, Miyake H, Yoshimura Y
  • Organizer: The 6th International Seminar on Tropical Animal Production (ISTAP)
  • Place of Presentation: Gadjah Mada University (Yogyakarta, Indonesia）
  • Year and Date: 2015-10-20
  • Int'l Joint Research
• [Presentation] Reaction of Cathelicidin-2 secreted from goats milk leukocytes to lipopolysaccharide. (2015)
  • Author(s): Nishikawa M, Yoshimura Y, Isobe N
  • Organizer: The 6th International Seminar on Tropical Animal Production (ISTAP)
  • Place of Presentation: Gadjah Mada University （Yogyakarta, Indonesia）
  • Year and Date: 2015-10-20
  • Int'l Joint Research
• [Presentation] ヤギ乳中白血球におけるカテリシジン-7の発現とそのリポ多糖に対する作用． (2015)
  • Author(s): 西川萌美・吉村幸則・磯部直樹
  • Organizer: 第20回日本乳房炎研究会学術集会
  • Place of Presentation: 国立科学博物館(東京都・台東区)
  • Year and Date: 2015-10-09
• [Presentation] Estradiolはウシの乳量を減少させ乳汁中の抗菌因子濃度を上昇させる． (2015)
  • Author(s): 三浦千佳・吉村幸則・磯部直樹
  • Organizer: 第20回日本乳房炎研究会学術集会
  • Place of Presentation: 国立科学博物館(東京都・台東区)
  • Year and Date: 2015-10-09
• [Presentation] 乳牛の高体細胞数乳汁における保存中生菌数の変化と抗菌因子との関係． (2015)
  • Author(s): 磯部直樹・久枝啓一・輿石智子・渡部雅子・三宅肇・吉村幸則
  • Organizer: 日本畜産学会第120回大会
  • Place of Presentation: 酪農学園大学(北海道・札幌市)
  • Year and Date: 2015-09-11
• [Presentation] リポ多糖の血中投与がヤギ乳房炎に及ぼす影響． (2015)
  • Author(s): 植田丈・吉村幸則・磯部直樹
  • Organizer: 第65回関西畜産学会大会
  • Place of Presentation: 愛媛大学（愛媛県・松山市）
  • Year and Date: 2015-09-03
• [Presentation] ウシ乳腺における水チャネルおよび密着結合分子の発現に及ぼすEstradiolの影響． (2015)
  • Author(s): 三浦千佳・吉村幸則・磯部直樹
  • Organizer: 第65回関西畜産学会大会
  • Place of Presentation: 愛媛大学（愛媛県・松山市）
  • Year and Date: 2015-09-03
• [Remarks] 広島大学大学院生物圏科学研究科家畜生体機構学研究室
  • URL: http://home.hiroshima-u.ac.jp/anat/index.html
• [Remarks] 広島大学大学院生物圏科学研究科家畜生体機構学研究室
  • URL: http://home.hiroshima-u.ac.jp/anat/
• [Remarks] 家畜生体機構学
  • URL: http://home.hiroshima-u.ac.jp/anat/