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Development of activation method for LH and FSH secretion in dairy cows based on pure bovine gonadotroph model

Research Project

Project/Area Number 15K07693
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Animal production science
Research InstitutionYamaguchi University

Principal Investigator

Kadokawa Hiroya  山口大学, 共同獣医学部, 准教授 (80370592)

Co-Investigator(Kenkyū-buntansha) 水上 洋一  山口大学, 大学研究推進機構, 教授 (80274158)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsウシ / ゴナドトロフ / 受容体 / 下垂体
Outline of Final Research Achievements

We aimed to determine gene expression patterns in the anterior pituitary (AP) of heifers before and after ovulation via deep sequencing of the transcriptome (RNA-seq) to identify new genes and clarify important pathways. The bovine APs expressed 12,769 annotated genes at pre- or post-ovulation. The bovine AP showed differential expression of 396 genes (P<0.05) in the pre- and post-ovulation APs. The 396 genes included new G-protein-coupled receptor (GPCR) genes and binding proteins. The AP also expressed 259 receptor and other 364 binding proteins. Moreover, ingenuity pathway analysis for the 396 genes revealed a canonical pathway linking GPCR to gene expression. Thus, the present study clarified the novel genes found to be differentially expressed before and after ovulation and clarified an important pathway in the AP. Some of the new GPCR colocalizes with GnRH receptor on the lipid raft in the plasma membrane of gonadotrophs.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (8 results)

All 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (4 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (1 results) (of which Int'l Joint Research: 1 results) Patent(Industrial Property Rights) (2 results)

  • [Int'l Joint Research] Universit&agrave; degli Studi di Milano(イタリア)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Anti-M&uuml;llerian hormone receptor type 2 is expressed in gonadotrophs of post-pubertal heifers to control gonadotropin secretion.2018

    • Author(s)
      Kereilwe Onalenna, Pandey Kiran, Borromeo Vitaliano, Kadokawa Hiroya.
    • Journal Title

      Reproduction, Fertility, and Development

      Volume: 印刷中 Issue: 9 Pages: 1192-1192

    • DOI

      10.1071/rd17377

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Deep sequencing of the transcriptome in the anterior pituitary of heifers before and after ovulation2017

    • Author(s)
      Pandey, K., Mizukami, Y., Watanabe, K., Sakaguti, S., Kadokawa, H.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 79 Issue: 6 Pages: 1003-1012

    • DOI

      10.1292/jvms.16-0531

    • NAID

      130005695792

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Heifers express G-protein coupled receptor 61 in anterior pituitary gonadotrophs in stage-dependent manner.2017

    • Author(s)
      Pandey Kiran, Kereilwe Onalenna, Borromeo Vitaliano, and Kadokawa Hiroya.
    • Journal Title

      Animal Reproduction Science

      Volume: 181 Pages: 93-102

    • DOI

      10.1016/j.anireprosci.2017.03.020

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Heifers express G-protein coupled receptor 153 in anterior pituitary gonadotrophs in stage-dependent manner.2017

    • Author(s)
      Pandey Kiran, Kereilwe Onalenna, and Kadokawa Hiroya.
    • Journal Title

      Animal Science Journal

      Volume: 89 Issue: 1 Pages: 60-71

    • DOI

      10.1111/asj.12920

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] Both estrone and estriol non-genomically suppress luteinizing hormone secretion from the bovine anterior pituitary cells via the estradiol receptor GPR30.2016

    • Author(s)
      Hiroya Kadokawa and Midori Otsuka
    • Organizer
      17th Animal Science Congress of AAAP
    • Place of Presentation
      Kyushu Sangyo University (Fukuoka, Fukuoka)
    • Year and Date
      2016-08-22
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] 卵胞刺激ホルモン分泌促進剤2017

    • Inventor(s)
      角川博哉
    • Industrial Property Rights Holder
      山口大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-212350
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 卵胞刺激ホルモン分泌促進剤2017

    • Inventor(s)
      角川博哉
    • Industrial Property Rights Holder
      山口大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-231717
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report

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Published: 2015-04-16   Modified: 2023-03-23  

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