Study of placenta releasing mechanism in cows and its practical application.
| Project/Area Number |
15K07700
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Animal production science
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
KAMADA HACHIRO 国立研究開発法人農業・食品産業技術総合研究機構, 畜産研究部門, 主席研究員 (70360443)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ウシ / 胎盤 / オキソアラキドン酸 / 栄養膜細胞 / 胎盤剥離 / 乳牛 / 重層構造 |
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| Outline of Final Research Achievements |
The response of trophoblast cells derived from bovine blastocyst against 12-KETE (a candidate of signal for placenta release at delivery) was investigated. Addition of 12-KETE (40μM) to the cell colony of trophoblast induced a rapid exfoliation into a cell sheet. In this process, matrix metalloproteinase (MMP) activation and apoptosis induction were observed. These results are consistent with in vivo observations, and strongly support the idea that 12-KETE is a placenta releasing signal. On the other hand, a low concentration (５-10μM) of 12-KETE addition induced a spheronization and subsequent floating of trophoblast. This reaction was suppressed by protease inhibitor. Activation of protease by low 12-KETE may propose the new idea on the placenta releasing mechanism at delivery.
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Report
(4 results)
Research Products
(4 results)
