Detection of miRNA associated with pork marbling
Project/Area Number |
15K07714
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Animal production science
|
Research Institution | National Agriculture and Food Research Organization (2016-2017) 国立研究開発法人農業生物資源研究所 (2015) |
Principal Investigator |
Taniguchi Masaaki 国立研究開発法人農業・食品産業技術総合研究機構, 畜産研究部門, 主任研究員 (60531431)
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 遺伝子発現調節 / マイクロRNA / バイオマーカー / 脂肪細胞分化 / ブタ / 霜降り肉 |
Outline of Final Research Achievements |
To identify molecular markers which are associated with IMF content in pork carcass, we aimed to detect differentially expressed genes including functional genes and microRNA (miRNA) genes, and to investigate relationships of those in longissimus dorsi (LD) muscle. Pork carcasses were produced by Landrace and Large White crossbred sows mated with a novel Duroc strain producing highly marbled carcass. IMF content was measured using Soxhlet extraction apparatus. Simultaneous gene expression analyses were conducted using Agilent microarrays for 44K messenger-RNA (mRNA) specific to pig genome and 80K micro-RNA (miRNA) including all pig and human miRNAs available from public databases. The miRNA array detected four significant miRNAs which were all upregulated in High IMF carcasses. Additionally, six functional genes were detected as downregulated genes in High IMF carcasses. The result suggests that miR-373-5p might be available molecular marker to estimate IMF content of pork meat.
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Report
(4 results)
Research Products
(1 results)