Research of viral community for unexplained livestock diarrhea and discovery of novel gastrointestinal viruses

• Project/Area Number: 15K07718
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Ishikawa Prefectural University (2016-2017); Tokyo University of Agriculture and Technology (2015)
• Principal Investigator: NAGAI Makoto 石川県立大学, 生物資源環境学部, 教授 (10540669)
• Co-Investigator(Kenkyū-buntansha): 青木 博史 日本獣医生命科学大学, 獣医学部, 准教授 (10440067)
• Co-Investigator(Renkei-kenkyūsha): MIZITANI Tetsuya 東京農工大学, 農学部, 教授 (70281681) ; SHIRAI Junsuke 東京農工大学, 連合農学院研究科, 教授 (00355182)
• Research Collaborator: Ito Mika ; Masuda Tsuneyuki ; Akagami Masataka ; Niira Kazutaka
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 家畜 / 下痢症 / ウイルス / メタゲノム解析 / 新種ウイルス / 遺伝子組み換え / 種間伝播 / 新規ウイルス / 分子疫学

Outline of Final Research Achievements

To clarify viral community for unexplained livestock (cattle and pig) diarrhea and discover novel gastrointestinal viruses, bovine and porcine fecal samples were analyzed using a metagenomics approach. We identified a novel picornavirus and new genotypes of Posavirus. Further studies for pathogenicity of these viruses are needed. Multiple possible recombination events in toroviruses, astroviruses, kobuviruses, enterovirueses, and sapoviruses were identified, suggesting that recombination might have contributed to the serological diversity and played an important role in the continued infection and the evolution of viruses in the farms.

Research Products

• [Journal Article] Genetic diversity and recombination of enterovirus G strains in Japanese pigs: High prevalence of strains carrying a papain-like cysteine protease sequence in the enterovirus G population (2018)
  • Author(s): Tsuchiaka S., Naoi Y., Imai R., Masuda T., Ito M., Akagami M., Ouchi Y., Ishii K., Sakaguchi S., Omatsu T., Katayama Y., Oba M., Shirai J., Satani Y., Takashima Y., Taniguchi Y., Takasu M., Madarame H., Sunaga F., Aoki H., Makino S., Mizutani T., Nagai M.
  • Journal Title: PLOS ONE Volume: 13 Issue: 1 Pages: e0190819-e0190819
  • DOI: 10.1371/journal.pone.0190819
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Diversity in VP3, NSP3, and NSP4 of rotavirus B detected from Japanese cattle. (2017)
  • Author(s): Hayashi-Miyamoto M, Murakami T, Minami-Fukuda F, Tsuchiaka S, Kishimoto M, Sano K, Naoi Y, Asano K, Ichimaru T, Haga K, Omatsu T, Katayama Y, Oba M, Aoki H, Shirai J, Ishida M, Katayama K, Mizutani T, Nagai M.
  • Journal Title: Infection, genetics and evolution Volume: 49 Pages: 97-103
  • DOI: 10.1016/j.meegid.2017.01.003
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Whole genome analysis of porcine astroviruses detected in Japanese pigs reveals genetic diversity and possible intra-genotypic recombination. (2017)
  • Author(s): Ito M, Kuroda M, Masuda T, Akagami M, Haga K, Tsuchiaka S, Kishimoto M, Naoi Y, Sano K, Omatsu T, Katayama Y, Oba M, Aoki H, Ichimaru T, Mukono I, Ouchi Y, Yamasato H, Shirai J, Katayama K, Mizutani T, Nagai M.
  • Journal Title: Infection, genetics and evolution Volume: 50 Pages: 38-48
  • DOI: 10.1016/j.meegid.2017.02.008
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Complete genome analysis of porcine kobuviruses from the feces of pigs in Japan (2017)
  • Author(s): Akagami Masataka、Ito Mika、Niira Kazutaka、Yamasato Hiroshi、Ouchi Yoshinao、Shirai Junsuke、Katayama Kazuhiko、Mizutani Tetsuya、Nagai Makoto
  • Journal Title: Virus Genes Volume: 53 Issue: 4 Pages: 593-602
  • DOI: 10.1007/s11262-017-1464-9
  • Peer Reviewed
• [Journal Article] Genetic diversity and intergenogroup recombination events of sapoviruses detected from feces of pigs in Japan (2017)
  • Author(s): Kuroda Moegi、Masuda Tsuneyuki、Ito Mika、Shirai Junsuke、Katayama Kazuhiko、Mizutani Tetsuya、Oka Tomoichiro、Nagai Makoto
  • Journal Title: Infection, Genetics and Evolution Volume: 55 Pages: 209-217
  • DOI: 10.1016/j.meegid.2017.09.013
  • Peer Reviewed
• [Journal Article] Identification of further diversity among posaviruses. (2016)
  • Author(s): Sano K, Naoi Y, Kishimoto M, Masuda T, Tanabe H, Ito M, Niira K, Haga K, Asano K, Tsuchiaka S, Omatsu T, Furuya T, Katayama Y, Oba M, Ouchi Y, Yamasato H, Ishida M, Shirai J, Katayama K, Mizutani T, Nagai M.
  • Journal Title: Archives of virology Volume: 161 Issue: 12 Pages: 3541-3548
  • DOI: 10.1007/s00705-016-3048-8
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Complete sequencing of bovine viral diarrhea virus 1 subgenotype 1n and 1o. (2016)
  • Author(s): Sato A, Tateishi K, Shinohara M, Naoi Y, Shiokawa M, Aoki H, Ohmori K, Mizutani T, Shirai J, Nagai M.
  • Journal Title: Genome announcements Volume: 18 Issue: 1 Pages: 1-1
  • DOI: 10.1128/genomea.01744-15
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Whole genome analysis of Japanese bovine toroviruses reveals natural recombination between porcine and bovine toroviruses. (2016)
  • Author(s): Ito M, Tsuchiaka S, Naoi Y, Otomaru K, Sato M, Masuda T, Haga K, Oka T, Yamasato H, Omatsu T, Sugimura S, Aoki H, Furuya T, Katayama Y, Oba M, Shirai J, Katayama K, Mizutani T, Nagai M.
  • Journal Title: Infection, genetics and evolution Volume: 38 Pages: 90-95
  • DOI: 10.1016/j.meegid.2015.12.013
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Characterization and phylogenetic analysis of a novel picornavirus from swine feces in Japan. (2016)
  • Author(s): Naoi Y, Kishimoto M, Masuda T, Ito M, Tsuchiaka S, Sano K, Yamasato H, Omatsu T, Aoki H, Furuya T, Katayama Y, Oba M, Okada T, Shirai J, Mizutani T, Nagai M.
  • Journal Title: Archives of virology Volume: in press
  • Peer Reviewed / Acknowledgement Compliant