Countermeasure against infectious source of human ascarid larval migrans: Investigation on the epidemiological role of cattle and chickens in southern Kyushu for effective control
| Project/Area Number |
15K07723
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
丸山 治彦 宮崎大学, 医学部, 教授 (90229625)
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| Co-Investigator(Renkei-kenkyūsha) |
Yoshida Ayako 宮崎大学, 農学部, 准教授 (20343486)
Misawa Naoaki 宮崎大学, 農学部, 教授 (20229678)
Uemura Ryoko 宮崎大学, 農学部, 助教 (90529190)
Horii Yoichiro 宮崎大学, 農学部, 名誉教授 (80173623)
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| Research Collaborator |
Maeda Chiori 宮崎県, 福祉保健部, 主任技師
Nguyen Thi Hoang Yen 宮崎大学, 医学獣医学総合研究科, 院生
Hayata Yayoi 宮崎大学, 農学部, 学生
Tanaka Shun 宮崎大学, 農学部, 学生
Sonoda Shino 宮崎大学, 農学部, 学生
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 動物由来回虫 / 犬回虫 / 猫回虫 / 豚回虫 / 鶏 / 牛 / 食品由来感染症 / 人獣共通感染症 |
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| Outline of Final Research Achievements |
As diagnostic tools for detecting the causative round worms of human ascarid larval migrans in chickens and cattle, we developed ELISAs for detecting specific antibodies against Ascaris suum and Toxocara spp. (T. canis and T. cati). Using the tools, we conducted an epidemiological study and found that the causative round worms such as A. suum, T. canis and T. cati would be endemic in some of chicken and cattle farms. In addition, as new tools required for further epidemiological investigation, we developed real-time PCR for detecting A. suum DNA and nested multiplex PCR for differentiating A. suum, T. canis and T. cati.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Evaluation of real-time PCR assay for the detection of Ascaris suum contamination in meat and organ meats.2017
Author(s)
Nguyen, Y.T.H., Wang, Z., Maruyama, H., Horii, Y., Nonaka, N., Yoshida, A.
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Journal Title
J. Food Safety
Volume: 37
Issue: 2
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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