| Project/Area Number |
15K07728
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | Nihon University |
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Principal Investigator |
SUGIYA Hiroshi 日本大学, 生物資源科学部, 教授 (20050114)
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| Co-Investigator(Renkei-kenkyūsha) |
NARITA Takanori 日本大学, 生物資源科学部, 講師 (70453884)
OKABAYASHI Ken 日本大学, 生物資源科学部, 講師 (20409072)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 炎症制御 / セラミド代謝物 / 炎症性サイトカイン / COX-2 / MAPキナーゼ / NF-κB / PGE2 / イヌ線維芽細胞 / TNF-α / IL-8 / ERK / JNK / siRNA / 線維芽細胞 / 炎症 / プロスタグランジンE2 / シクロオキシゲナーゼ2 / インターロイキン1β |
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| Outline of Final Research Achievements |
To elucidate the inflammatory regulation by ceramide metabolites such as ceramide 1-phosphate (C-1-P) and sphingosine 1-phosphate (S-1-P), IL-1β-mediated PGE2 production system in canine dermal fibroblasts (cDFB) and TNF-α-mediated IL-8 expression system in canine synovial fibroblasts (cSFB) were established as a model of inflammation first. In cDFB, IL-1β mediated PGE2 production via COX-2 expression. MEK/ERK MAPK signaling was involved in the IL-1β-mediated COX-2 expression via the activation of NF-κB (p65), a transcription factor. In cSFB, ERK2 and JNK1, ERK and JNK MAPK isoforms, respectively, were involved in the TNF-α-mediated IL-8 expression. Then the effects of C-1-P and S-1-P on IL-1β-mediated PGE2 production in cDFB and TNF-α-mediated IL-8 expression in cSFB was examined. However, no significant effect of these ceramide metabolites was observed.
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