Studies on phenotypes of Listeria monocytogens grown under room temperature and refrigeration conditions
Project/Area Number |
15K07729
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Veterinary medical science
|
Research Institution | Nippon Veterinary and Life Science University |
Principal Investigator |
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | リステリア / 酸 / 過酸化水素 / 温度 / ストレス |
Outline of Final Research Achievements |
Listeria monocytogenes is a food-borne pathogen. In the present study, L. monocytogenes grown at 20℃ or 37℃ was subjected to low pH and hydrogen peroxide under different temperatures, including 20℃ and 37℃. Survival curves indicated an increase in the resistance of L. monocytogenes to hydrogen peroxide as the temperature decreased during the procedure of stress exposure. In addition, transcriptional induction of some genes that have central roles in response to hydrogen peroxide exposure, including kat and sigB, was observed at 37℃ but not at 20℃, whereas other stress response genes that were involved in gene expression during the stress exposure were induced at the both temperatures. These findings suggest that hydrogen peroxide is not recognized as a potent stress factor under room temperature conditions. Therefore, this study suggests that this pathogen exerts the high ability to survive and propagate in food processing environments.
|
Report
(4 results)
Research Products
(6 results)
-
[Journal Article] Sequential transition of the injury phenotype, temperature-dependent survival and transcriptional response in Listeria monocytogenes following lethal H2O2 exposure.2017
Author(s)
Ochiai, Y., Yamada, F., Yoshikawa, Y., Mochizuki, M., Takano, T., Hondo, R., and Ueda, F.
-
Journal Title
International Journal of Food Microbiology
Volume: 259
Pages: 52-58
DOI
Related Report
Peer Reviewed
-
[Journal Article] Genetic subtyping of <i>Listeria monocytogenes</i> via multiple-locus sequence typing using <i>iap, sigB</i> and <i>actA</i>2016
Author(s)
Yoshikawa, Y., Ochiai, Y., Mochizuki, M., Fujita, O., Takano, T., Hondo, R., and Ueda, F.
-
Journal Title
Journal of Veterinary Medical Science
Volume: 78
Issue: 12
Pages: 1831-1839
DOI
NAID
ISSN
0916-7250, 1347-7439
Related Report
Peer Reviewed
-
-
-
-