| Project/Area Number |
15K07747
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
Hatoya Shingo 大阪府立大学, 生命環境科学研究科, 准教授 (40453138)
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| Co-Investigator(Kenkyū-buntansha) |
杉浦 喜久弥 大阪府立大学, 生命環境科学研究科, 教授 (30171143)
竹中 重雄 大阪府立大学, 総合リハビリテーション学研究科, 教授 (10280067)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKANISHI Mahito 国立研究開発法人産業技術総合研究所, その他部局等, 研究員 (10172355)
HISASUE Masaharu 麻布大学, 獣医学部, 准教授 (80333144)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | iPS細胞 / 多能性幹細胞 / 再生医療 / 獣医学 / イヌ / ネコ / 血液 / センダイウイルスベクター / 獣医再生医療 / 再生獣医療 |
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| Outline of Final Research Achievements |
The objective of this study was to establish genome integration-free canine and feline induced pluripotent stem cells (ciPSCs), and to induce hematopoietic cells from these stem cells. The following results were obtained.
①We confirmed that the Sendai virus vector (SeVdp) could infect canine and feline embryonic fibroblasts by detecting GFP expression. ②We could reprogram feline embryonic fibroblasts into iPS-like cells using SeVdp containing 6 transcription factors with TGF-beta inhibitor. These cells were positive for pluripotent markers. ③We transduced four transcriptional factors into canine fibroblast cells with SeVdp, and could established iPS-like cell line using TGF-beta inhibitor. Finally, we reprogrammed canine embryonic fibroblasts using the auto-erasable defective and persistent SeVdp, and canine iPS-like cells were formed. Further studies are required to induce hematopoietic cells from these canine or feline stem cells.
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