| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Outline of Final Research Achievements |
To clarify morphology and physiology of chemoreceptor cell-nerve ending complex in the laryngeal mucosa, immunohistochemistry, Ca2+ imaging and electrophysiological analysis were performed. In the present study, GNAT3-immunoreactive (ir) chemoreceptor cells were observed in the laryngeal mucosa as the solitary chemoreceptor cells (SCC) and the taste bud-like chemosensory cell clusters (CC). The SCC were mainly observed in the mocosa covered with epiglottic and arytenoid cartilages, and the CC were observed in the epiglottis and aryepiglottic fold. The GNAT3-ir cells were attached with the P2X3-ir nerve endings. By calcium imaging, some epithelial cells in the laryngeal mucosa evoked intracellular Ca2+ by 10 mM quinine. Application of quinine to the laryngeal mucosa decreased respiration frequency. It is suggest that the laryngeal chemosensory cell-nerve ending complex involve with perception of chemical substances in the laryngeal cavity to evoke the reflex for respiratory depression.
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