Roles of membrane rafts in molecular basis of fertilition in avian sperm
| Project/Area Number |
15K07761
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Integrative animal science
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| Research Institution | University of Tsukuba |
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Principal Investigator |
ASANO Atsushi 筑波大学, 生命環境系, 助教 (10630981)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 精子 / 先体反応 / 鳥類 / 細胞内シグナリンク / 細胞膜マイクロドメイン / ステロール / 細胞膜 / プロテオミクス / シグナリング / 膜ラフト / AMPK / グルコース / 精子ー卵認識 / 細胞内シグナリング / 受精 |
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| Outline of Final Research Achievements |
①Qualitative analysis of functional roles of membrane rafts was conducted using quantitative proteomics combined with dimethyl-labeling approach, demonstrating at least 82 proteins are relatively or exclusively enriched in membrane rafts.
②Membrane rafts regulate adenyl cyclase isoforms, such as sAC and tmAC, and glucose transporter activities, thereby stimulating acrosome reaction induction in chicken sperm.
③ Above results led to development of avian sperm cryopreservation and demonstrated that adding sterols into the plasma membranes of chicken sperm dramatically improves the post-thaw semen quality.
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Report
(4 results)
Research Products
(20 results)
