Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Outline of Final Research Achievements |
①Qualitative analysis of functional roles of membrane rafts was conducted using quantitative proteomics combined with dimethyl-labeling approach, demonstrating at least 82 proteins are relatively or exclusively enriched in membrane rafts. ②Membrane rafts regulate adenyl cyclase isoforms, such as sAC and tmAC, and glucose transporter activities, thereby stimulating acrosome reaction induction in chicken sperm. ③ Above results led to development of avian sperm cryopreservation and demonstrated that adding sterols into the plasma membranes of chicken sperm dramatically improves the post-thaw semen quality.
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