Genome-wide epigenetic analysis of cloned cattle from the same donor cells

• Project/Area Number: 15K07764
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Kaneda Masahiro 東京農工大学, (連合)農学研究科(研究院), 准教授 (80469840)
• Research Collaborator: KIEFER Helene ; JOUNEAU Luc ; JAMMES Hélène ; BALZERGUE Sandrine
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 体細胞クローン牛 / エピジェネティクス / DNAメチル化 / MeDIP-chip / インプリント遺伝子

Outline of Final Research Achievements

Genome-wide epigenetic analysis focusing on DNA methylation levels were conducted using tissues from cloned cattle derived from the same donor cells. Five cloned cattle from the same donor cells and five non-cloned cattle were used for the analysis. Genomic DNA was extracted from liver and MeDIP (Methylated DNA Immunoprecipitation)-chip method was used for DNA methylation analysis. The correlation clustering showed that the difference among individual cattle was larger in non-cloned group compared to cloned group.

Research Products

• [Int'l Joint Research] INRA(フランス)
• [Int'l Joint Research] INRA(フランス)
• [Journal Article] Proper reprogramming of imprinted and non-imprinted genes in cloned cattle gametogenesis (2017)
  • Author(s): Kaneda Masahiro、Watanabe Shinya、Akagi Satoshi、Inaba Yasushi、Geshi Masaya、Nagai Takashi
  • Journal Title: Animal Science Journal Volume: 88 Issue: 11 Pages: 1678-1685
  • DOI: 10.1111/asj.12846
  • Peer Reviewed
• [Journal Article] Epigenetic analysis of bovine parthenogenetic embryonic fibroblasts (2017)
  • Author(s): Masahiro Kaneda, Masashi Takahishi, Ken-ichi Yamanaka, Koji Saito, Masanori Taniguchi, Satoshi Akagi, Shinya Watanabe, Takashi Nagai
  • Journal Title: Journal of Reproduction and Development Volume: 63 Issue: 4 Pages: 365-375
  • DOI: 10.1262/jrd.2017-040
  • NAID: 130005982812
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed
• [Presentation] Epigenetic analysis of bovine parthenogenetic embryonic fibroblasts (2016)
  • Author(s): 金田正弘
  • Organizer: 新学術領域研究“生殖細胞のエピゲノムダイナミクスとその制御”国際シンポジウム
  • Place of Presentation: 京都大学
  • Year and Date: 2016-02-17
  • Int'l Joint Research