Elucidation of muscarinic receptor subtypes in regulatory mechanism on ATP, NO and SP releasing nerves in the large intestine

• Project/Area Number: 15K07765
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: Gifu University
• Principal Investigator: Matsuyama Hayato 岐阜大学, 応用生物科学部, 准教授 (80345800)
• Co-Investigator(Kenkyū-buntansha): 齋藤 正一郎 岐阜大学, 応用生物科学部, 准教授 (60325371) ; 海野 年弘 岐阜大学, 応用生物科学部, 教授 (90252121)
• Research Collaborator: TANAHASHI Yasuyuki ; SUZUKI Kasumi ; ITO Atsushi ; SAKUMA Emi ; NAGANO Hiroshi ; FIROJ Alom
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 神経 / ムスカリン受容体 / 内臓平滑筋 / ムスカリン受容体サブタイプ / 腸神経―平滑筋接合部電位 / 収縮 / 視床下部 / バソプレシン / 腸神経 / 平滑筋 / 情報伝達

Outline of Final Research Achievements

Among M1 to M5 muscarinic receptor subtypes, M2 and M3 receptors are located to not only a smooth muscle cell but also various enteric nerve cells. In this study, to make clear the mechanisms of peristalsis of the large intestine, we have investigated the role of M2 and M3 receptors on the enteric nerves, using muscarinic receptor subtypes knockout mice. In consequence of the study, both M2 and M3 receptor subtypes enhanced the ATP-mediated neuromuscular transmissions in the smooth muscle cells in the large intestine. In addition, M2 receptor-mediated contraction was larger than M3-mediated one. Furthermore, it is also expected that novel signaling molecules may be involved in the both M2 and M3 receptor-mediated contractions in the large intestine.

Research Products

• [Journal Article] Muscarinic M2 receptor promotes vasopressin synthesis in mice supraoptic nuclei. (2018)
  • Author(s): Nagano, H., Sobue, Y., Matsuyama, M., Saito, S., Sakai, H., Alom, F., Tanahashi, Y., Ishii, T. and Unno, T.
  • Journal Title: Journal of Endocrinology Volume: － Issue: 2 Pages: 207-216
  • DOI: 10.1530/joe-17-0630
  • Peer Reviewed
• [Presentation] Possible involvement of TRPM4 channels in the cholinergic contractile responses in mouse detrusor and ileal smooth muscles (2018)
  • Author(s): Firoj Alom, Hayato Matsuyama, Hiroshi Nagano, Yasuyuki Tanahashi, Toshihiro Unno
  • Organizer: 第91回日本薬理学会年会、第18回国際薬理学・臨床薬理学会議
  • Int'l Joint Research
• [Presentation] マウス結腸輪走筋のコリン作動性神経ー平滑筋伝達におけるM2およびM3ムスカリン受容体サブタイプの役割 (2017)
  • Author(s): 松山勇人、佐久間絵光、永野宏、棚橋靖行、北澤多喜雄、海野年弘
  • Organizer: 第160回日本獣医学会学術集会
• [Presentation] マウス大腸輪走筋におけるコリン作動性収縮は主にM2ムスカリン受容体サブタイプにより仲介される (2016)
  • Author(s): 鈴木香澄，佐久間絵光，松山勇人，棚橋靖行，永野宏，北澤多喜雄，Jurgen Wess，小森成一，海野年弘
  • Organizer: 日本薬理学会
  • Place of Presentation: パシフィコ横浜 会議センター
  • Year and Date: 2016-03-10
• [Presentation] ムスカリン受容体を介した胃粘膜細胞の分化・増殖制御 (2016)
  • Author(s): 松山勇人、内藤清惟、和田善明、永野宏、齋藤正一郎、酒井洋樹、棚橋靖行、北澤多喜雄、小森成一、海野年弘
  • Organizer: 第159回日本獣医学会
  • Place of Presentation: 日本大学 生物資源科学部（湘南キャンパス）
• [Book] 獣医臨床薬理学 (2017)
  • Author(s): 日本比較薬理学・毒性学会
  • Total Pages: 240
  • Publisher: 近代出版
  • ISBN: 9784874022382
• [Remarks] 岐阜大学 獣医薬理学研究室のホームページへようこそ！！
  • URL: http://www1.gifu-u.ac.jp/~pharmaco/