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Molecular dissection of sexual dimorphic metamorphosis in scale insects

Research Project

Project/Area Number 15K07791
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Insect science
Research InstitutionNagoya University

Principal Investigator

MINAKUCHI Chieka  名古屋大学, 生命農学研究科, 講師 (90509134)

Co-Investigator(Renkei-kenkyūsha) 小野 肇  京都大学, 農学研究科, 助教
宮下 正弘  京都大学, 農学研究科, 准教授
新美 輝幸  基礎生物学研究所, 教授
Research Collaborator Vea Isabelle  University of Illinois at Chicago, 博士研究員
篠田 徹郎  農研機構, ユニット長
塩月 孝博  農研機構, 上級研究員
Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords幼若ホルモン / カイガラムシ / 性的二型 / 脱皮ホルモン
Outline of Final Research Achievements

Scale insects have evolved extreme sexual dimorphism, as demonstrated by juvenile-like (or neotenic) females and winged males. This study aimed to elucidate the mechanism of sexual determination/differentiation in scale insects, as well as hormonal regulation of sexual dimorphic metamorphosis. We revealed that an adult specifier E93 is expressed exclusively in male metamorphosis, while female development proceeds with near absence of E93. Sequence analysis of the 5’ regulatory region of E93 identified the presence of Kruppel homolog 1 binding site as well as doublesex binding site. These results indicated a crosstalk between hormonal cascade and sexual determination/differentiation cascade.

Academic Significance and Societal Importance of the Research Achievements

昆虫の性特異的形質の発達におけるホルモンの役割に関しては、昆虫全般を見渡してもほとんど研究例がなく、本研究は強いインパクトを与える先駆的なものである。また本研究の成果は、カイガラムシの特殊な変態がどのように進化したかという謎の解明につながるものであり、生物学における学術的な意義が極めて大きい。さらに、本研究で得られた知見を利用すれば、世界的な農業害虫であるカイガラムシ類のホルモン作用をかく乱することで致死効果をもたらすような新規殺虫剤の開発が可能になると考えられ、将来的には植物保護に大きく貢献すると期待できる。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (16 results)

All 2019 2018 2017 2016

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results)

  • [Journal Article] E93 expression and links to the juvenile hormone in hemipteran mealybugs with insights on female neoteny2019

    • Author(s)
      Vea Isabelle Mifom、Tanaka Sayumi、Tsuji Tomohiro、Shiotsuki Takahiro、Jouraku Akiya、Minakuchi Chieka
    • Journal Title

      Insect Biochemistry and Molecular Biology

      Volume: 104 Pages: 65-72

    • DOI

      10.1016/j.ibmb.2018.11.008

    • NAID

      120006957714

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 害虫防除への応用を目指した、昆虫ホルモン作用の基礎研究2019

    • Author(s)
      水口智江可
    • Journal Title

      Comparative Endocrinology

      Volume: 45 Issue: 166 Pages: 39-39

    • DOI

      10.5983/nl2008jsce.45.39

    • NAID

      130007614038

    • ISSN
      1882-6636, 1882-6644
    • Related Report
      2018 Annual Research Report
  • [Journal Article] Differential Juvenile Hormone Variations in Scale Insect Extreme Sexual Dimorphism2016

    • Author(s)
      Vea IM, Tanaka S, Shiotsuki T, Jouraku A, Tanaka T, Minakuchi C
    • Journal Title

      PLOS One

      Volume: 11 (2) Issue: 2 Pages: e0149459-e0149459

    • DOI

      10.1371/journal.pone.0149459

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Elucidate the Roles of E75 during Pupal-Adult Metamorphosis of Red Flour Beetle, Tribolium castaneum2019

    • Author(s)
      Sapin G, Tomoda K, Miura K, Minakuchi C
    • Organizer
      第63回日本応用動物昆虫学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 性的二型を示すフジコナカイガラムシにおける脱皮ホルモン生合成酵素遺伝子の発現解析2019

    • Author(s)
      村松美幸・Isabelle Vea・田中彩友美・辻知広・三浦健・水口智江可
    • Organizer
      第63回日本応用動物昆虫学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ホソヘリカメムシにおける幼若ホルモン様活性物質の殺卵活性2019

    • Author(s)
      成瀬祥矢・塩月孝博・上樂明也・三浦健・水口智江可
    • Organizer
      第63回日本応用動物昆虫学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 害虫防除への応用を目指した、昆虫ホルモン作用の基礎研究2018

    • Author(s)
      水口智江可
    • Organizer
      2018「第43回日本比較内分泌学会大会およびシンポジウム 仙台大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] ホソヘリカメムシの産卵と胚発生における幼若ホルモン受容体の機能解析2018

    • Author(s)
      成瀬祥矢、三浦健、水口智江可
    • Organizer
      第62回日本応用動物昆虫学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] フジコナカイガラムシの顕著な性的二型形成における幼若ホルモンの役割2017

    • Author(s)
      田中彩友美、Isabelle Vea、三浦健、水口智江可
    • Organizer
      第61回日本応用動物昆虫学会大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2017-03-27
    • Related Report
      2016 Research-status Report
  • [Presentation] フジコナカイガラムシにおけるエクダイソン初期応答遺伝子E75のcDNAクローニングと発現解析2017

    • Author(s)
      辻知広、Isabelle Vea、塩月孝博、上樂明也、三浦健、水口智江可
    • Organizer
      第61回日本応用動物昆虫学会大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2017-03-27
    • Related Report
      2016 Research-status Report
  • [Presentation] Hormonal regulation of metamorphosis in hemimetabolous thrips and scale insects2017

    • Author(s)
      Vea IM, Tanaka S, Tsuji T, Suzuki Y, Shiotsuki T, Jouraku A, Miura K and Minakuchi C
    • Organizer
      The 3rd International Insect Hormone Workshop 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] dentification and expression profiles of sex-specific doublesex transcripts in the non-holometabolous scale insects (Hemiptera: Coccomorpha)2016

    • Author(s)
      Isabelle Vea, Sumika Yonei, Takahiro Shiotsuki, Akiya Jouraku, Teruyuki Niimi, Chieka Minakuchi
    • Organizer
      25th International Congress of Entomology
    • Place of Presentation
      Orlando, FL
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Role of broad in scale insect juvenile hormone signaling during male quiescent stages2016

    • Author(s)
      Isabelle Vea, Sayumi Tanaka, Takahiro Shiotsuki, Akiya Joraku, Chieka Minakuchi
    • Organizer
      日本昆虫学会第76回大会・第60回日本応用動物昆虫学会大会合同大会
    • Place of Presentation
      大阪府立大学
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] フジコナカイガラムシにおける幼若ホルモンシグナル伝達関連因子TaimanのcDNA単離および発現解析2016

    • Author(s)
      田中彩友美、Isabelle Vea, 塩月孝博、上樂明也、三浦健、水口智江可
    • Organizer
      日本昆虫学会第76回大会・第60回日本応用動物昆虫学会大会合同大会
    • Place of Presentation
      大阪府立大学
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] Role of the Juvenile Hormone in establishing scale insect extreme sexual dimorphism2016

    • Author(s)
      Isabelle Vea, Sayumi Tanaka, Takahiro Shiotsuki, Akiya Joraku, Toshiharu Tanaka, Chieka Minakuchi
    • Organizer
      Society for Integrative and Comparative Biology Annual Meeting 2016
    • Place of Presentation
      Oregon Convention Center
    • Year and Date
      2016-01-03
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Scale insect development and the adult specifier E93: towards an understanding of female neoteny2016

    • Author(s)
      Isabelle Vea, Sayumi Tanaka, Takahiro Shiotsuki, Akiya Joraku, Chieka Minakuchi
    • Organizer
      Society for Integrative and Comparative Biology Annual Meeting 2016
    • Place of Presentation
      Oregon Convention Center
    • Year and Date
      2016-01-03
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2020-03-30  

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