| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
(1) The objective of this study was to evaluate the major basic protein profile of Bombyx seminal fluid and to determine if any of these proteins was associated with semen fertility. Six candidate proteins included Arg-Arg extended sequences in common. Moreover, they exhibited a step-wise regression with the progress of sperm maturation. These findings suggested that mainly the specific cleavage at Arg-Arg motif is caused by a trypsin-type protease, called initiatorin, which is thought to be involved in the sperm maturation. (2) We indicate that flagellar beating of silkworm sperm was controlled by Ca2+ levels, cAMP-dependent protein kinases (PKA) activities and the specific degradation of threonine-phosphorylated protein.
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