Protein modules by recombinant silkworms

• Project/Area Number: 15K07794
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Insect science
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: Mori Hajime 京都工芸繊維大学, その他部局等, 副学長 (80201812)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: カイコ / ウイルス / 多角体 / 細胞増殖因子 / 多層構造 / プロテインモジュール / 遺伝子組換えカイコ / piggyBac / FGF-7 / シルクガット

Outline of Final Research Achievements

PC12 cells have been widely used as a model for neural differentiation. When PC12 cells are cultured in a medium containing nerve growth factor (NGF), neuronal differentiation is known to be induced. NGF-encapsulated polyhedra were spotted onto a collagen-coated cover slip and PC12 cells were seeded and cultured for 5 days without medium exchange. Then, interestingly, alignment of PC12cells along the periphery of the polyhedron spot field was observed. Also, axons were extended from PC12 cells and each cell was connected via the extended axons. These phenomena were not seen with a commercially supplied soluble NGF and normal polyhedra. The alignment of PC12 cells was suggested to be induced by a gradient of NGF concentration from a spot of NGF-encapsulated polyhedra. This study suggested that NGF-encapsulated polyhedra mimic the extracellular matrix in which NGF are retained and secreted and finally contribute to nerve regeneration.

Research Products

• [Journal Article] Structure of in cell protein crystals containing organometallic complexes (2018)
  • Author(s): Abe Satoshi、Atsumi Kohei、Yamashita Keitaro、Hirata Kunio、Mori Hajime、Ueno Takafumi
  • Journal Title: Physical Chemistry Chemical Physics Volume: 20 Issue: 5 Pages: 2986-2989
  • DOI: 10.1039/c7cp06651a
  • Peer Reviewed
• [Journal Article] Supramolecular protein cages constructed from a crystalline protein matrix (2018)
  • Author(s): Negishi Hashiru、Abe Satoshi、Yamashita Keitaro、Hirata Kunio、Niwase Kento、Boudes Marion、Coulibaly Fasseli、Mori Hajime、Ueno Takafumi
  • Journal Title: Chemical Communications Volume: 54 Issue: 16 Pages: 1988-1991
  • DOI: 10.1039/c7cc08689j
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Crystal Engineering of Self-Assembled Porous Protein Materials in Living Cells (2017)
  • Author(s): S. Abe, H. Tabe, H. Ijiri, K. Yamashita, K. Hirata, K. Atsumi, T. Shimoi, M. Akai, H. Mori, S. Kitagawa and T. Ueno
  • Journal Title: ACS Nano Volume: 11 Issue: 3 Pages: 2410-2419
  • DOI: 10.1021/acsnano.6b06099
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Bioengineered silkworms with butterfly cytotoxin-modified silk glands produce sericin cocoons with a utility for a new biomaterial. (2017)
  • Author(s): Otsuki, R., Yamamoto, M., Matsumoto, E., Iwamoto, S., Sezutsu, H., Suzui, M., Takaki, K., Wakabayashi, K., Mori, H., Kotani, E.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 114 Issue: 26 Pages: 6740-6745
  • DOI: 10.1073/pnas.1703449114
  • Peer Reviewed
• [Journal Article] Bioengineered silkworms with butterfly cytotoxin-modified silk glands produce sericin cocoons with a utility for a new biomaterial (2017)
  • Author(s): Otsuki, R., Yamamoto, M., Matsumoto, E., Iwamoto, S., Sezutsu, H., Suzui, M., Takaki, K., Wakabayashi, K., Mori, H., Kotani, E.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Design of Enzyme-Encapsulated Protein Containers by in Vivo Crystal Engineering (2015)
  • Author(s): Abe, S., Ijiri, H., Negishi, H., Yamanaka, H., Sasaki, K., Hirata, K., Mori, H., Ueno, T.
  • Journal Title: Adv. Mater. Volume: 27 Issue: 48 Pages: 7951-7956
  • DOI: 10.1002/adma.201503827
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cell proliferation by silk gut incorporating FGF-2 protein microcrystals. (2015)
  • Author(s): Kotani, E., Yamamoto, N., Kobayashi, I., Uchino, K., Muto, S., Ijiri1, H., Shimabukuro, J., Tamura, T., Sezutsu, H., Mori, H.
  • Journal Title: Sci. Rep. Volume: 5 Issue: 1 Pages: 1-10
  • DOI: 10.1038/srep11051
  • Peer Reviewed / Open Access / Acknowledgement Compliant