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Control of the proprerties of silk materials by the introduction of unnatural amino acids

Research Project

Project/Area Number 15K07800
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Insect science
Research InstitutionNational Agriculture and Food Research Organization (2016-2017)
国立研究開発法人農業生物資源研究所 (2015)

Principal Investigator

Teramoto Hidetoshi  国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門 新産業開拓研究領域 新素材開発ユニット, 上級研究員 (60391562)

Co-Investigator(Kenkyū-buntansha) 玉田 靖  信州大学, 学術研究院繊維学系, 教授 (70370666)
Co-Investigator(Renkei-kenkyūsha) KOJIMA Katsura  国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門新産業開拓研究領域新素材開発ユニット, 上級研究員 (40370655)
NAKAJIMA Ken-ichi  国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門新産業開拓研究領域新特性シルク開発ユニット, 主席研究員 (70391563)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsバイオテクノロジー / 非天然アミノ酸
Outline of Final Research Achievements

Adhesion of fibroblasts (NIH3T3) on silk fibroin, incorporated with an azide-bearing unnatural amino acid (AzPhe), was investigated after being modified with cell adhesive RGD peptides or cell repulsive polyethylene glycols (PEG). When the RGD peptide was ligated on silk fibroin, fibroblasts with extended forms became abundant although number of adhered cells did not increase. When the PEG chain was ligated on silk fibroin, number of adhered cells significantly decreased. It was demonstrated that cell patterning on silk fibroin is possible by partially destroying azide groups by UV irradiation.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (26 results)

All 2018 2017 2016 2015 Other

All Journal Article (7 results) (of which Open Access: 2 results,  Peer Reviewed: 6 results,  Acknowledgement Compliant: 4 results) Presentation (17 results) (of which Int'l Joint Research: 3 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Genetic Code Expansion of the Silkworm Bombyx mori to Functionalize Silk Fiber2018

    • Author(s)
      Teramoto H, Amano Y, Iraha F, Kojima K, Ito T, Sakamoto K
    • Journal Title

      ACS Synthetic Biology

      Volume: 7 Issue: 3 Pages: 801-806

    • DOI

      10.1021/acssynbio.7b00437

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 非天然型アミノ酸の導入によるシルクの改変:クリッカブルシルクの創製2017

    • Author(s)
      寺本英敏
    • Journal Title

      蚕糸・昆虫バイオテック

      Volume: 86 Pages: 25-38

    • NAID

      130007411792

    • Related Report
      2017 Annual Research Report
    • Open Access
  • [Journal Article] In vivo Incorporation of an Alkyne-Bearing Amino Acid into Bombyx mori Silk Fibroin2017

    • Author(s)
      Hidetoshi Teramoto
    • Journal Title

      Journal of Insect Biotechnology and Sericology

      Volume: 86 Issue: 3 Pages: 3_113-3_121

    • DOI

      10.11416/jibs.86.3_113

    • NAID

      130006223755

    • ISSN
      1346-8073, 1884-7978
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Intermolecular Crosslinking of Silk Fibroin by Click Chemistry2017

    • Author(s)
      寺本 英敏
    • Journal Title

      The Journal of Silk Science and Technology of Japan

      Volume: 25 Issue: 0 Pages: 17-25

    • DOI

      10.11417/silk.25.17

    • NAID

      130005450601

    • ISSN
      1880-8204, 1881-1698
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Inhibitory effects on silk fibroin production by the expression of phenylalanyl-tRNA synthetase mutants in posterior silk glands of Bombyx mori2016

    • Author(s)
      Hidetoshi Teramoto
    • Journal Title

      Journal of Insect Biotechnology and Sericology

      Volume: 85(2) Pages: 31-37

    • NAID

      130005421098

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Azide-Incorporated Clickable Silk Fibroin Materials with the Ability to Photopattern2016

    • Author(s)
      Hidetoshi Teramoto, Ken-ichi Nakajima, and Katsura Kojima
    • Journal Title

      ACS Biomater. Sci. Eng.

      Volume: 2(2) Issue: 2 Pages: 251-258

    • DOI

      10.1021/acsbiomaterials.5b00469

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Click Modification of Azide-Incorporated Silk Fibroin Film2016

    • Author(s)
      寺本 英敏, 佐々木 瑞樹, 玉田 靖, 小島 桂
    • Journal Title

      The Journal of Silk Science and Technology of Japan

      Volume: 24 Issue: 0 Pages: 33-36

    • DOI

      10.11417/silk.24.33

    • NAID

      130005139794

    • ISSN
      1880-8204, 1881-1698
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] クリック反応によるシルク基盤上への細胞パターニング2018

    • Author(s)
      寺本英敏、白川美徳、玉田 靖
    • Organizer
      つくば医工連携フォーラム2018
    • Related Report
      2017 Annual Research Report
  • [Presentation] クリッカブルシルク:「結合の手」をもつシルク素材の開発2018

    • Author(s)
      寺本英敏
    • Organizer
      アグリテクノフェア in 北海道
    • Related Report
      2017 Annual Research Report
  • [Presentation] 機能分子を簡単につなげられる「結合の手」を もつシルクの高効率生産を実現2018

    • Author(s)
      寺本英敏、小島 桂、坂本健作
    • Organizer
      農研機構プレスリリース
    • Related Report
      2017 Annual Research Report
  • [Presentation] アジド基をもつ非天然型アミノ酸を効率よくフィブロインに導入できる遺伝子組換えカイコの作出2018

    • Author(s)
      寺本英敏、小島 桂、坂本健作、天野芳美、伊良波史枝、伊藤拓宏
    • Organizer
      平成30年度蚕糸・昆虫機能利用学術講演会(日本蚕糸学会第88回大会)
    • Related Report
      2017 Annual Research Report
  • [Presentation] クリック反応によるフィブロインフィルムの表面修飾と細胞付着性評価2018

    • Author(s)
      寺本英敏、白川美徳、玉田 靖
    • Organizer
      第65回日本シルク学会研究発表会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Genetic code expansion of the silkworm Bombyx mori to enhance the utility of silk for biomedical applications2018

    • Author(s)
      Hidetoshi Teramoto, Yoshimi Amano, Fumie Iraha, Minori Shirakawa, Katsura Kojima, Takuhiro Ito, Yasushi Tamada, Kensaku Sakamoto
    • Organizer
      2018 Genetic Code Expansion Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] アルキン基を有する非天然型アミノ酸を導入したフィブロインの作出2017

    • Author(s)
      寺本英敏
    • Organizer
      平成29年度蚕糸・昆虫機能利用学術講演会(日本蚕糸学会第87回大会)
    • Place of Presentation
      農林水産技術会議事務局筑波産学連携支援センター(茨城県・つくば市)
    • Year and Date
      2017-03-21
    • Related Report
      2016 Research-status Report
  • [Presentation] クリック反応によるフィブロインへの RGDペプチド固定化条件の検討2017

    • Author(s)
      寺本英敏、佐々木瑞樹、白川美徳、玉田 靖
    • Organizer
      第64回日本シルク学会研究発表会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シルク・フィブロインの化学修飾を目的としたカイコBombyx moriにおける遺伝暗号の拡張2017

    • Author(s)
      坂本健作、天野芳美、伊良波史枝、伊藤拓宏、小島 桂、寺本英敏
    • Organizer
      第12回無細胞生命科学研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] クリッカブルシルク:望みの機能を簡単に付加できる次世代バイオ繊維2016

    • Author(s)
      寺本英敏
    • Organizer
      アグリビジネス創出フェア2016
    • Place of Presentation
      東京ビッグサイト(東京都・江東区)
    • Year and Date
      2016-12-14
    • Related Report
      2016 Research-status Report
  • [Presentation] フィブロインに導入したアジド基を起点とする架橋形成の試み2016

    • Author(s)
      寺本英敏,小島桂,中島健一
    • Organizer
      第63回日本シルク学会研究発表会
    • Place of Presentation
      東京農工大学小金井キャンパス(東京都・小金井市)
    • Year and Date
      2016-07-22
    • Related Report
      2016 Research-status Report
  • [Presentation] Clickable silk: novel silk materials containing azide reactive groups2016

    • Author(s)
      Hidetoshi Teramoto, Mizuki Sasaki, Yasushi Tamada, Kenichi Nakajima and Katsura Kojima
    • Organizer
      10th World Biomaterials Congress
    • Place of Presentation
      The Palais des congres de Montreal, Montreal (カナダ)
    • Year and Date
      2016-05-19
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] クリッカブルシルク:望む機能を自在に付加できるタンパク質材料2016

    • Author(s)
      寺本英敏
    • Organizer
      SATテクノロジー・ショーケース 2016
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-02-04
    • Related Report
      2015 Research-status Report
  • [Presentation] クリッカブルシルク:アジド基導入によりクリック修飾と光パターニングが可能なシルクタンパク質材料2016

    • Author(s)
      寺本英敏, 佐々木瑞樹, 玉田靖, 中島健一, 小島桂
    • Organizer
      つくば医工連携フォーラム2016
    • Place of Presentation
      産業技術総合研究所(茨城県つくば市)
    • Year and Date
      2016-01-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Transgenic silkworms expressing phenylalanyl-tRNA synthetase mutants in their silk glands produce silk fiber incorporated with unnatural amino acids2015

    • Author(s)
      Teramoto H, Kojima K
    • Organizer
      10th International Symposium on Aminoacyl-tRNA Synthetases
    • Place of Presentation
      スペイン、バルセロナ
    • Year and Date
      2015-10-21
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] アミノ酸認識能を拡張したフェニルアラニル-tRNA合成酵素を後部絹糸腺で発現する遺伝子組換えカイコの性状2015

    • Author(s)
      寺本英敏, 小島桂, 中島健一
    • Organizer
      平成27年度蚕糸・昆虫機能利用学術講演会
    • Place of Presentation
      北海道大学農学部(札幌市)
    • Year and Date
      2015-09-27
    • Related Report
      2015 Research-status Report
  • [Presentation] クリック反応によるアジド基導入フィブロインフィルムの修飾2015

    • Author(s)
      寺本英敏, 小島桂, 玉田靖
    • Organizer
      第62回日本シルク学会研究発表会
    • Place of Presentation
      岡谷商工会議所(長野県岡谷市)
    • Year and Date
      2015-05-21
    • Related Report
      2015 Research-status Report
  • [Remarks] (研究成果) 機能分子を簡単につなげられる「結合の手」を もつシルクの高効率生産を実現

    • URL

      http://www.naro.affrc.go.jp/publicity_report/press/laboratory/nias/079965.html

    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] トランスジェニックカイコ、および該カイコを用いた非天然アミノ酸含有タンパク質の製造方法2018

    • Inventor(s)
      寺本英敏、小島 桂、坂本健作、木村史枝、山口芳美
    • Industrial Property Rights Holder
      農研機構、理化学研究所
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2017 Annual Research Report
    • Overseas

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Published: 2015-04-16   Modified: 2019-03-29  

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