Production of affinity silk materials for targeting to influenza virus

Research Project

Project/Area Number	15K07801
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Insect science
Research Institution	National Agriculture and Food Research Organization (2016-2017) 国立研究開発法人農業生物資源研究所 (2015)
Principal Investigator	Kojima Katsura 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 上級研究員 (40370655)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	アフィニティーシルク / scFv / 遺伝子組換えカイコ / シルク / フィブロイン / ELISA / インフルエンザウイルス / ScFv
Outline of Final Research Achievements	We generated a transgenic silkworm expressing a cDNA construct containing fibroin L-chain fused to a single-chain variable fragment (scFv)derived from aMAb against influenza virus. The transgenic cocoons were dissolved in aqueous lithium bromide solution, the specific binding of scFv-conjugated affinity silk solution for the target influenza virus protein was verified by ELISA. We demonstrated that the transgenic silk protein could be used as antibody for capturing and detecting influenza virus protein in ELISA system.