Development of optically active lactic acid producing system from non-sterilized by-product of the rice as single raw material using the environment-conscious bioprocess.
| Project/Area Number |
15K07809
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental agriculture(including landscape science)
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Research Collaborator |
SASANO Kazuo
KASHIWAMURA Takashi
SHIONO Tadahiko
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 光学活性乳酸 / 米加工副産物 / 菌体外プロテアーゼ / T-RFLP法 / 米由来バイオマス / 髙光学純度乳酸 / 同時糖化発酵 / ポリ乳酸 / T-RFLP解析 / 高光学純度乳酸 |
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| Outline of Final Research Achievements |
In this study, we found that extracellular protease activity of a high lactic acid producing bacterium Lactobacillus rhamnosus M-23 was relatively higher than the other isolated LABs from a rice washing drainage storage tank. The results of the T-RFLP analysis showed that SSF culture inoculated with strain M-23 was dominated by Clostridium sp. cluster IX and XIVa at an early stage of SSF culture, respectively. In comparison with an inoculation of relatively low lactic acid producing strain, Clostridium sp. cluster IX mainly dominated with the high accumulation of VFAs (acetic and propionic acid) and the decrease of produced lactic acid in SSF culture compared with the case of strain M-23 inoculation. These results demonstrated the synergetic effect of proteinaceous material removal of the starch particle surface by extracellular protease as well as a repressive effect of dominance formation of Clostridium sp. cluster IX in SSF culture.
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Report
(4 results)
Research Products
(16 results)
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[Presentation] Characterization of lipase from thermotolerant Streptomyces thermoviolaceus strain TCW2016
Author(s)
S.Takenaka, U.Osaka, A.Kuntiya, C.Techapun, N. Leksawasdi, P.Seesuriyachan, M.Watanabe, T.Chaiyaso
Organizer
JSPS Core to core program A (CCP), Advanced research networks, the 2nd Joint seminar
Place of Presentation
Bangsaen heritage hotel (Chonburi, Thailand）
Year and Date
2016-11-14
Related Report
Int'l Joint Research / Invited
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[Presentation] Purification and characterization of thermostable cellulase-free endo-xylanase from Streptomyces thermovulgaris TISTR1948 and its application on xylooligosaccharide production2016
Author(s)
T.Chaiyaso, P. Boonchuay, S.Takenaka, M.Watanabe, A.Kuntiya, C.Techapun, N. Leksawasdi, P.Seesuriyachan
Organizer
JSPS Core to core program A (CCP), Advanced research networks, the 2nd Joint seminar
Place of Presentation
Bangsaen heritage hotel (Chonburi, Thailand）
Year and Date
2016-11-14
Related Report
Int'l Joint Research / Invited
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