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Identification of genetic loci in relation to salt tolerance of rice using wild rice and development of breeding basis toward the production of resistant cultivars

Research Project

Project/Area Number 15K07810
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental agriculture(including landscape science)
Research InstitutionShinshu University

Principal Investigator

Horie Tomoaki  信州大学, 学術研究院繊維学系, 准教授 (90591181)

Co-Investigator(Kenkyū-buntansha) 石川 亮  神戸大学, 農学研究科, 助教 (70467687)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords耐塩性 / イネ / 遺伝資源
Outline of Final Research Achievements

Phenotypic evaluation for the sensitivity to salt stress has led to the findings that Oryza rufipogon, which is an ancestral wild rice to the cultivated rice (Oryza sativa), showed higher salt sensitivity with significantly higher Na accumulation in leaves compared with a japonica rice cultivar Nipponbare under salt stress. We have analyzed concentrations of key elements, Na, K, Ca and Mg, in leaves under salt stress using backcross recombinant inbred lines (BRILs), produced by crossing Nipponbare (recurrent) with wild rice (donor). Based on the data of leaf Na accumulation from 152 lines, three important candidates regions for QTL were identified. In addition, genetic regions, which could include QTL for the control of the accumulation of K, Ca, or Mg, were also narrowed down.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (4 results)

All 2018 2017 Other

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (1 results) Remarks (1 results)

  • [Journal Article] T-DNA Tagging-Based Gain-of-Function of OsHKT1;4 Reinforces Na Exclusion from Leaves and Stems but Triggers Na Toxicity in Roots of Rice Under Salt Stress2018

    • Author(s)
      Yuuka Oda, Natsuko I. Kobayashi, Keitaro Tanoi, Jian Feng Ma, Yukiko Itou, Maki Katsuhara, Takashi Itou. Tomoaki Horie
    • Journal Title

      Int. J. Mol. Sci.

      Volume: 19 Issue: 1 Pages: 235-235

    • DOI

      10.3390/ijms19010235

    • NAID

      120007099652

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genomics, Physiology, and Molecular Breeding Approaches for Improving Salt Tolerance.2017

    • Author(s)
      Ismail AM and Horie T
    • Journal Title

      Annual Review of Plant Biology

      Volume: 68 Issue: 1 Pages: 405-434

    • DOI

      10.1146/annurev-arplant-042916-040936

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 野生イネ Oryza rufipogon と栽培イネ Oryza sativa の交雑系統群を利用した新規 Na+ 輸送系遺伝子・ 変異の探索2017

    • Author(s)
      堀内智貴、石川亮、田野井慶太朗、小林奈通子、石井尊生、堀江智明
    • Organizer
      日本育種学会 第132回講演会
    • Related Report
      2017 Annual Research Report
  • [Remarks] 信州大学学術オンラインシステムSOAR

    • URL

      http://soar-rd.shinshu-u.ac.jp/profile/ja.OFTmWUDU.html

    • Related Report
      2017 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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