| Project/Area Number |
15K07811
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental agriculture(including landscape science)
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| Research Institution | Gifu University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 青枯病 / 萎凋病 / 生物防除 / 内生細菌 / 茎葉散布 / Bacillus / トマト / 地上部処理 / 土壌病害 |
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| Outline of Final Research Achievements |
The aim of this study was to develop a novel microbial pesticide which can control soil-borne diseases by foliar application. For this purpose, we recovered 83 isolates of endophytic Bacillus spp. from tomato plants. By the preliminary screening using a tomato seedling assay, we selected 7 isolates, which showed >60% protection against tomato bacterial wilt (TBW), as primary biocontrol candidates. Subsequently, we tested the biocontrol effect of foliar application of the candidate isolates in pot experiments. As a result, an isolate GEBT457 showed the highest and stable suppressive effect against TBW. Accordingly, the isolate GEBT457 was selected as a final candidate. In the field experiments, foliar spraying of the isolate GEBT457 suppressed wilt incidence by about 40-50%, suggesting that this isolate was effective even under the field conditions. The result of gene expression analysis indicated that the candidate isolate induced systemic resistance in tomato plants.
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