Analysis and production method of mycovirus protein having broad antibacterial spectrum
| Project/Area Number |
15K07838
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Moriyama Hiromitsu 東京農工大学, (連合)農学研究科(研究院), 准教授 (20392673)
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| Co-Investigator(Kenkyū-buntansha) |
川本 進 千葉大学, 真菌医学研究センター, 客員教授 (80125921)
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| Research Collaborator |
Takahashi Azusa
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | マイコウイルス / 2本鎖RNA / 酵母異種発現系 / イネいもち病菌 / アルターナリア属菌 / 微生物培養 / タンパク質産生 / ウイルス粒子精製 / 植物病原菌 / 酵母異種発現 / 生物防除資材 / 2本鎖RNAウイルス / 生育阻害 / 生育促進 / 弱毒化ウイルス / 生育阻害タンパク質 / 生育促進タンパク質 / タンパク質機能解析 / アルタ―ナリア・アルタナータ菌 / 生理活性タンパク質 / 物質生産 / 抗菌性タンパク質 |
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| Outline of Final Research Achievements |
MoCV1-A-derived ORF4 protein (811 aa) and AaCV1-derived ORF2 protein (776 aa) have 18.7% identity and 62.9% similarity, and both expression in yeast cells causes growth inhibition It became clear. As a result of RNA-Seq analysis, in yeast cells in which growth inhibition was observed due to MoCV1-A ORF4 expression, the expression level of the stress response gene was reduced by a factor of 5 to 20, or conversely It was found that the expression of genes involved in ergosterol resistance was increased. In addition, amino acid sequences involved in growth promotion were also newly identified, and mechanical analysis was started.
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| Academic Significance and Societal Importance of the Research Achievements |
植物病原菌にマイコウイルスが感染すると、宿主菌の病原性を弱めたり（弱毒化）、逆に強めたり（強毒化）する性質が付与されることがある。本研究において、研究代表者らはイネいもち病菌マイコウイルス(MoCV1-A)とAlternaria alternata菌マイコウイルス(AaCV1)由来で遺伝子配列情報だけでは機能が未知であるタンパク質の生物的活性を、パン酵母異種発現系を利用することにより、顕在化し得る評価系を改良し確立できた。
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Report
(5 results)
Research Products
(62 results)
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[Journal Article] Molecular characterization of a novel mycovirus in Alternaria alternata manifesting two-sided effects: down-regulation of host growth and up-regulation of host plant pathogenicity.2018
Author(s)
Okada R, Ichinose S, Takeshita K, Urayama S, Fukuhara T, Komatsu K, Arie T, Ishihara A, Egusa M, Kodama M, Moriyama H（責任著者）
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Journal Title
Virology
Volume: 458
Pages: 265-273
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Magnaporthe oryzae chrysovirus 1 strain A infection triggers reduced virulence and pathogenic race conversion of the host fungus, Magnaporthe oryzae.2018
Author(s)
Aihara M, Urayama S, Le TM, Katoh T, Higashiura T, Fukuhara T, Arie T, Teraoka T, Komatsu K, Moriyama H
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Journal Title
Journal of General Plant Pathology
Volume: 84
Issue: 2
Pages: 92-103
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Identification, characterization, and full-length sequence analysis of a novel double-stranded RNAvirus isolated from an arboreal ant, Camponotus yamaokai.2015
Author(s)
Koyama S, Urayama SI, Ohmatsu T, Sassa Y, Sakai C, Takata M, Hayashi S, Nagai M, Furuya T, Moriyama H, Satoh T, Ono SI, Mizutani T.
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Journal Title
J Gen Virol.
Volume: 96
Issue: 7
Pages: 1930-1937
DOI
Related Report
Peer Reviewed
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[Presentation] Fungicide sensitivity of Japanese isolates of an asparagus Phytophthora rot pathogen infected with an endornavirus and trial for isolation of virus-free isolates.2017
Author(s)
Uchida,K.,Takahashi,Y.,Okada,R.,Fukuhara,T.,Arie,T.,Teraoka,T.,Uematsu,S., Moriyama,H.
Organizer
The 4th International Mycovirus Symposium
Related Report
Int'l Joint Research
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[Presentation] Magnaporthe oryzae chrysovirus 1 strain A infection triggers reduced virulence and pathogenic race conversion of the host fungus, Pyricularia oryzae2017
Author(s)
Ken Komatsu, Mitsuhiro Aihara, Yuta Owashi, Syun-ichi Urayama, Tuong Le Minh, Yu Katoh, Tomoya Higashiura, Toshiyuki Fukuhara, Tsutomu Arie, Tohru Teraoka, Hiromitsu Moriyama
Organizer
The 4th International Mycovirus Symposium
Related Report
Int'l Joint Research
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