Functions of secreted proteins to establish a symbiotic association with its host plant in fungal endophyte
Project/Area Number |
15K07839
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied molecular and cellular biology
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Research Institution | Nagoya University |
Principal Investigator |
Takemoto Aiko 名古屋大学, 生命農学研究科, 学振特別研究員(RPD) (90464148)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 共生 / 転写制御因子 |
Outline of Final Research Achievements |
ProA is a transcription factor necessary for maintaining a symbiotic relationship with the host grass plants in Epichloe festucae. To identify ProA-binding sequences across the genome, we used a gSELEX method and collected the genomic DNA pools after gSELEX selection. The genomic DNA pools were sequenced using a Miseq platform. The direct target sequences were identified by mapping the reads to a reference sequence. Functional analysis of the potential new targets for ProA revealed 4 genes are encoding the proteins that contribute to maintaining the proper symbiotic interaction between E. festucae and the host ryegrass plants.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] SymB and SymC, two membrane associated proteins, are required for Epichloe festucae hyphal cell-cell fusion and maintenance of a mutualistic interaction with Lolium perenne2017
Author(s)
Green K, Becker Y, Tanaka A, Takemoto D, Fitzsimons H, Seiler S, Lalucque H, Silar P, Scott B
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Journal Title
Molecular Microbiology
Volume: 103
Issue: 4
Pages: 657-677
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] Novel nuclear protein, NsiA, and its interacting transcription factor Ste12 and MAP kinase Mak2 mediate balanced symbiotic infection of endophytic fungus Epichoe festucae in perennial ryegrass2016
Author(s)
Kamiya S., Ozaki Y., Okamura A., Kameoka S., Kayano Y., Saikia S., Scott B., Maruyama J., Tanaka A. and Takemoto D.
Organizer
XVII International Congress on Molecular Plant-Microbe Interactions
Place of Presentation
Portland, Oregon, USA
Year and Date
2016-07-17
Related Report
Int'l Joint Research
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