Development of the modified ubiquitin ligase to degrade of a pathogenic protein
| Project/Area Number |
15K07840
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied molecular and cellular biology
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
黒田 俊一 大阪大学, 産業科学研究所, 准教授 (60263406)
藤井 郁雄 大阪府立大学, 理学(系)研究科(研究院), 教授 (70189984)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 病原タンパク質 / タンパク質分解 / ユビキチンシステム / ドラッグデリバリーシステム / 一細胞 |
|---|
| Outline of Final Research Achievements |
As a substrate recognition site of a modified-ubiquitin ligase targeting a pathogenic protein, we aimed to screen an affibody from a library consisted of an antibody binding domain of protein A. Affibody-gene library was constructed from protein A of which some amino acid residues was randomized. On the other hand, as a modified ubiquitin ligase-delivery system, we modified the HBV envelope protein derived particle (bio-nanocapsule: BNC), and resulting antibody-associating BNC, LG-BNC, was expressed in yeast cells and purified. We also succeeded in expressing modified BNC as virus-like particles in E. coli. This particle might be a substitute for LG-BNC expressed in yeast.
|
Report
(4 results)
Research Products
(17 results)
