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Preparation of functional glyco-polymers and analysis of their cellular immune activities to develop anti-pollinosis drugs

Research Project

Project/Area Number 15K07841
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionOkayama University

Principal Investigator

MAEDA Megumi  岡山大学, 環境生命科学研究科, 准教授 (20434988)

Co-Investigator(Renkei-kenkyūsha) KIMURA Yoshinobu  岡山大学, 大学院環境生命科学研究科, 教授 (70195387)
OKANO Mitsuhiro  国際医療福祉大学, 医学部, 教授 (60304359)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords糖鎖ポリマー / 花粉アレルゲン / N-グリカン / 植物抗原性糖鎖 / ルイスa抗原 / Th2免疫応答 / 樹状細胞 / γ-ポリグルタミン酸 / HLA-DR / NFkB / Cry j1 / Cha o3 / Th1免疫応答 / IFN-γ産生 / ハイマンノース型糖鎖 / 動物複合型糖鎖 / スギ花粉症 / Th2
Outline of Final Research Achievements

Three types of glycopolymers bearing multivalent N-glycans (plant complex type, high-mannose type, or animal complex type) were synthesized. Their suppressive functions on Th2 cellular immune response induced by Japanese cedar pollen allergen, Cry j1, were analyzed by using dendritic cells. And then, structural features of newly identified Japanese cypress pollen allergen, Cha o3, were analyzed. The glycoform of Cha o 3 is similar to those of major glycoallergens in cedar or cypress pollens (Cry j1 and Jun a1).

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (33 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (3 results) Journal Article (9 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 8 results,  Open Access: 7 results,  Acknowledgement Compliant: 2 results) Presentation (18 results) (of which Int'l Joint Research: 6 results,  Invited: 1 results) Remarks (3 results)

  • [Int'l Joint Research] Department ofMolecular Biotechnology/Ghent university(Belgium)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] Applied Genetics and Cell Biology/University of Nat Resources and Life Sci(Austria)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] Biochemistry and Molecular Biology/University of Rajshahi(Bangladesh)

    • Related Report
      2016 Research-status Report
  • [Journal Article] スギ・ヒノキ花粉アレルゲンに結合したN-グリカンの構造特性と免疫活性解析に向けた新技術2018

    • Author(s)
      前田恵・木村万里子・木村吉伸
    • Journal Title

      アレルギーの臨床

      Volume: 38 Pages: 55-59

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans.2018

    • Author(s)
      Rahman Z, Tsujimori Y, Maeda M, Hossain A, Ishimizu T, Kimura Y.
    • Journal Title

      J Biochem.

      Volume: 印刷中 Issue: 1 Pages: 53-63

    • DOI

      10.1093/jb/mvy029

    • NAID

      40021625325

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Glycoform of a newly identified pollen allergen, Cha o 3, from Chamaecyparis obtusa (Japanese cypress, Hinoki).2017

    • Author(s)
      Osada T, Maeda M, Tanabe C, Furuta K, Vavricka CJ, Sasaki E, Okano M, Kimura Y.
    • Journal Title

      Carbohydr Res.

      Volume: 448 Pages: 18-23

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Structural feature of N-glycans of bamboo shoot glycoproteins: useful source of plant antigenic N-glycans2017

    • Author(s)
      Tanabe Chinatsu、Furuta Kaori、Maeda Megumi、Kimura Yoshinobu
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 81 Issue: 7 Pages: 1405-1408

    • DOI

      10.1080/09168451.2017.1320519

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Ginkgo biloba α-fucosidase with activity towards plant complex type N-glycans containing the Lewis a epitope: Purification and characterization.2017

    • Author(s)
      Itano S., Maeda M., Rahman M.Z., and Kimura Y.
    • Journal Title

      Scientific Reports of the Faculty of Agriculture, Okayama University

      Volume: 106 Pages: 5-12

    • NAID

      120005970803

    • Related Report
      2016 Research-status Report
    • Open Access
  • [Journal Article] Molecular characterization of tomato α1,3/4-fucosidase, a member of glycosyl hydrolase family 29, involved in degradation of plant complex type N-glycans.2017

    • Author(s)
      Rahman, M.D., Maeda, M., Itano, S., Hossain, M.A., Ishimizu, T., Kimura, Y.
    • Journal Title

      J. Biochem.

      Volume: 161 Pages: 421-432

    • DOI

      10.1093/jb/mvw089

    • NAID

      40021219805

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Structural features of N-glycans linked to glycoproteins expressed in three kinds of water plants: Predominant occurrence of the plant complex type N-glycans bearing Lewis a epitope.2016

    • Author(s)
      Maeda M., Tani M., Yoshiie T., Vavricka CJ., Kimura Y.
    • Journal Title

      Carbohydr Res.

      Volume: 435 Pages: 50-57

    • DOI

      10.1016/j.carres.2016.09.008

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Rice α-fucosidase active against plant complex type N-glycans containing Lewis a epitope: purification and characterization.2016

    • Author(s)
      Rahman MZ, Fujishige M, Maeda M, Kimura Y.
    • Journal Title

      Biosci Biotechnol Biochem.

      Volume: 80 Issue: 2 Pages: 291-294

    • DOI

      10.1080/09168451.2015.1079479

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] β-Galactosidase from Ginkgo biloba seeds active against β-galactose-containing N-glycans: Purification and characterization.2015

    • Author(s)
      Md. Ziaur Rahman, Megumi Maeda, and Yoshinobu Kimura
    • Journal Title

      Bioscence, Biotechnoligy, and Biochemistry

      Volume: 79 Issue: 9 Pages: 1-6

    • DOI

      10.1080/09168451.2015.1034653

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 抗原性糖鎖の多量調製法確立を目指したタケノコ糖タンパク質糖鎖の構造解析2018

    • Author(s)
      田邉千夏,古田佳織,前田恵,木村吉伸
    • Organizer
      日本農芸化学会中四国支部第50回記念講演会(例会)広島大学東広島キャンパス
    • Related Report
      2017 Annual Research Report
  • [Presentation] 多価糖鎖結合ポリマーがTh1,Th2免疫応答に及ぼす免疫活性の解析2017

    • Author(s)
      板野紗月,前田 恵,木村吉伸
    • Organizer
      支部創立15周年記念 日本農芸化学会中四国第47回講演会(支部例会)
    • Place of Presentation
      島根大学 松江キャンパス
    • Year and Date
      2017-01-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 新規ヒノキ花粉アレルゲンCha o3の糖鎖構造解析2017

    • Author(s)
      前田 恵,田邉千夏,長田年弘,佐々木英治,岡野光博,木村吉伸
    • Organizer
      支部創立15周年記念 日本農芸化学会中四国第47回講演会(支部例会)
    • Place of Presentation
      島根大学 松江キャンパス
    • Year and Date
      2017-01-28
    • Related Report
      2016 Research-status Report
  • [Presentation] MOLECULAR CHARACTERIZATION SOLANUM LYCOPERSICUM Α1,3/4-FUCOSIDASE II (Α-FUC’ASE SL-II) INVOLVED IN DEGRADATION OF PLANT COMPLEX TYPE N-GLYCAN2017

    • Author(s)
      Yuta Tsujimori , M. Rahman, M. Maeda, M. Hossain, Y. Kimura.
    • Organizer
      19th European Carbohydrate Symposium (EUROCARB), Barcelona CCIB, Spain
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 免疫活性N-グリカンを有する糖鎖ポリマーの合成と応用2017

    • Author(s)
      前田 恵
    • Organizer
      第58回日本生化学会中国・四国支部例会 シンポジウム「糖鎖認識の生化学」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Glycoform analysis of a new major allergen, Cha o3, from Japanese cypress pollen2017

    • Author(s)
      C. Tanabe, M. Maeda, T. Osada, E. Sasaki, M. Okano, Y. Kimura
    • Organizer
      12th Carbohydrate Bioengineering Meeting(The CBM12), Audi Max, Augasse 2-6, 1090 Vienna/Austria
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Immunomodulatory activity of glycopolymers bearing highly clustered N-glycans for Th1 and Th2 immune response.2016

    • Author(s)
      Satsuki Itano, Megumi Maeda, and Yoshinobu Kimura
    • Organizer
      第45回日本免疫学会学術集会
    • Place of Presentation
      沖縄コンベンションセンター,ラグナガーデンホテル
    • Year and Date
      2016-12-07
    • Related Report
      2016 Research-status Report
  • [Presentation] α1,3/4-フコシダーゼ欠損 Arabidopsis thalianaに存在する遊離糖鎖の構造解析2016

    • Author(s)
      前田 恵,髙田 駿,石水 毅,木村 吉伸
    • Organizer
      支部創立15周年記念 日本農芸化学会中四国支部第44回講演会(例会)
    • Place of Presentation
      岡山県立大学
    • Year and Date
      2016-01-23
    • Related Report
      2015 Research-status Report
  • [Presentation] イネ由来エンドグリコシダーゼ(Endo-Os)の糖転移活性解析2015

    • Author(s)
      瀬尾和子,前田恵,木村吉伸
    • Organizer
      第88回生化学会大会(BMB2015)
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-02
    • Related Report
      2015 Research-status Report
  • [Presentation] Lewis a抗原含有N-グリカンの多量精製及び糖鎖ポリマーの作製2015

    • Author(s)
      谷美里,前田恵,木村吉伸
    • Organizer
      第88回生化学会大会(BMB2015)
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-02
    • Related Report
      2015 Research-status Report
  • [Presentation] 遊離N-グリカンの生理機能解明を目的とした酸性ペプチドN-グリカナーゼ(aPNGase)の細胞内局在解析2015

    • Author(s)
      松丸千紘,前田恵,藤川真奈,中野龍平,梶浦裕之,三崎亮,藤山和仁,木村吉伸
    • Organizer
      第88回生化学会大会(BMB2015)
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] トマト酸性ペプチド: N-グリカナーゼの細胞内局在解析と過剰発現体の解析2015

    • Author(s)
      松丸千紘,前田恵,木村吉伸
    • Organizer
      第48 回おかやまバイオアクティブ研究会シンポジウム・第14回学生プレゼンテーション・ポスターセッション
    • Place of Presentation
      岡山県天神山文化プラザ
    • Year and Date
      2015-11-14
    • Related Report
      2015 Research-status Report
  • [Presentation] SYNTHESIS OF A NOVEL GLYCOPOLYMER CARRYING MULTIVALENT PLANT ANTIGENIC N-GLYCANS2015

    • Author(s)
      Megumi Maeda, Naoto Takeda, Mariko Kimura, Yoshinobu Kimura
    • Organizer
      23rd International Symposium on Glycoconjugates
    • Place of Presentation
      Split, Croatia
    • Year and Date
      2015-09-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] PURIFICATION AND CHARACTERIZATION OF GINKGO BILOBA 1,3/4-FUCOSIDASE (α-FUC’ASE GB) ACTIVE AGAINST LEWIS A ANTIGEN2015

    • Author(s)
      Satsuki Itano, Megumi Maeda, Yoshinobu Kimura
    • Organizer
      23rd International Symposium on Glycoconjugates
    • Place of Presentation
      Split, Croatia
    • Year and Date
      2015-09-16
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] CLONING AND EXPRESSION ANALYSIS OF ACIDIC PEPTIDE: N-GLYCANASE GENES IN SOLANUM LYCOPERSICUM2015

    • Author(s)
      Mana Fujikawa, Megumi Maeda, Chihiro Matsumaru, Shouehi Murata, Ryo Misaki, Kazuhito Fujiyama, Yoshinobu Kimura
    • Organizer
      23rd International Symposium on Glycoconjugates
    • Place of Presentation
      Split, Croatia
    • Year and Date
      2015-09-16
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] A possible processing pathway of free N-glycans (FNGs) formed in cytosol: Possibility of translocation of FNGs from cytosol to ER/Golgi.2015

    • Author(s)
      Megumi Maeda, Natsuki Ebara, Misato Tani, and Yoshinobu Kimura
    • Organizer
      the 4th Austria-Japan Seminar for Comparative and Developmental Glycobiology
    • Place of Presentation
      Split, Croatia
    • Year and Date
      2015-09-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 植物・動物複合型N-グリカンを多価に結合した糖鎖ポリマーの作製2015

    • Author(s)
      前田恵,竹田直人,木村吉伸
    • Organizer
      第34回日本糖質学会年会
    • Place of Presentation
      東京大学安田講堂・工学部・山上会館
    • Year and Date
      2015-08-02
    • Related Report
      2015 Research-status Report
  • [Presentation] 植物複合型N-グリカンの分解に関わるトマトα1,3/4-フコシダーゼの遺伝子クローニングと基質特異性解析2015

    • Author(s)
      モハマド ジアウール ラーマン, 前田 恵, モハマド アノワール ホサイン,木村吉伸
    • Organizer
      第34回日本糖質学会年会
    • Place of Presentation
      東京大学安田講堂・工学部・山上会館
    • Year and Date
      2015-08-02
    • Related Report
      2015 Research-status Report
  • [Remarks] 糖鎖機能化学研究室

    • URL

      http://www.okayama-u.ac.jp/user/agr/profile/nougaku01_3.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 岡山大学 研究者総覧

    • URL

      http://soran.cc.okayama-u.ac.jp/view?l=ja&u=ec49c68c89dc75d674506e4da22f6611#item_shuyogsk_highlight_2

    • Related Report
      2017 Annual Research Report
  • [Remarks] 岡山大学農学部糖鎖機能化学研究室

    • URL

      http://www.okayama-u.ac.jp/user/agr/profile/nougaku01_3.html

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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