| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
In this research, we aimed at installing cyanobacterial bicarbonate transporters into the inner envelope membrane of chloroplasts and creating "hybrid-chloroplast" carrying CO2-concentrating mechanism. We have successfully generated transgenic plants expressing chimeric cyanobacterial bicarbonate transporters (BicAI, BicAII, SbtAII, and SbtAIII). Furthermore, we have demonstrated that these chimeric bicarbonate transporters are localized to the inner envelope membrane of chloroplasts. We have also explored conditions to investigate bicarbonate transport activity using isolated chloroplasts.
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