Generation and characterization of transgenic plants carrying hybrid chloroplasts
Project/Area Number |
15K07843
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied molecular and cellular biology
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Research Institution | University of Miyazaki |
Principal Investigator |
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 葉緑体 / 光合成 / シロイヌナズナ / 重炭酸イオン輸送体 |
Outline of Final Research Achievements |
In this research, we aimed at installing cyanobacterial bicarbonate transporters into the inner envelope membrane of chloroplasts and creating "hybrid-chloroplast" carrying CO2-concentrating mechanism. We have successfully generated transgenic plants expressing chimeric cyanobacterial bicarbonate transporters (BicAI, BicAII, SbtAII, and SbtAIII). Furthermore, we have demonstrated that these chimeric bicarbonate transporters are localized to the inner envelope membrane of chloroplasts. We have also explored conditions to investigate bicarbonate transport activity using isolated chloroplasts.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Ubiquitin-proteasome dependent regulation of GLK1 in response to plastid signals in Arabidopsis.2017
Author(s)
Tokumaru, M., Adachi, F., Toda, M., Ito-Inaba, Y., Yazu, F., Hirosawa, Y., Sakakibara, Y., Suiko, M., Kakizaki, T., Inaba, T.
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Journal Title
Plant. Physiol.
Volume: 173
Issue: 1
Pages: 524-524
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Characterization of two PEBP genes, SrFT and SrMFT, in thermogenic skunk cabbage (Symplocarpus renifolius).2016
Author(s)
Ito-Inaba, Y., Masuko-Suzuki, H., Maekawa, H., Watanabe, M., and Inaba, T.
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Journal Title
Sci. Rep.
Volume: 6
Issue: 1
Pages: 29440-29440
DOI
Related Report
Peer Reviewed / Open Access
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