| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
High temperature is one of the most important abiotic stresses because it can greatly affect plant growth and crop production. Many accessions of Arabidopsis thaliana have large genetic variations in phenotypic traits such as stress tolerance. We previously performed QTL analysis using recombinant inbred lines generated by crossing between Da (1)-12 and Ei-2 and found a major QTL for heat tolerance on chromosome 1. To identify the QTL, we developed NILs which carried different sized small chromosomal segments from Ei-2 containing the QTL region in the genetic background of Da (1)-12. As a result of genotyping for the NILs, the QTL was narrowed down into 140 kbp including 34 genes.
HsfA1d was identified as a gene that was able to confer heat tolerance to plants. We here generated HsfA1d-overexpressing tomato plants. These HsfA1d-overexpressing tomato plants shows highly expression levels of heat-response marker genes including HSPs compared with the wild type plants.
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